Human peripheral blood lymphocytes were stained with purified mouse IgG1 anti-human CD3 (clone UCHT1, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) Brilliant Violet 421™.
Human peripheral blood lymphocytes were stained with purified mouse IgG1 anti-human CD3 (clone UCHT1, filled histogram) or mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG1 (clone RMG1-1) Brilliant Violet 421™.
The RMG1-1 monoclonal antibody reacts with immunoglobulin G1 (IgG1) in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References
(PubMed link indicates BioLegend citation)
Rucci F, et al. 2010. P. Natl. Acad. Sci. USA 107:3024. (FC) PubMed
Zhang JG, et al. 2011. Int. Immunopharmacol. 11:1685. PubMed
Product Citations
Stoler-Barak L, et al. 2023. Nat Commun. 14:1462. PubMed
van den Biggelaar RHGA, et al. 2020. Front Immunol. 11:141. PubMed
Lin H, et al. 2015. Mol Ther Methods Clin Dev. 2: 15046. PubMed
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