APC anti-mouse TER-119/Erythroid Cells Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Day-14 fetal liver cells from a C57BL/6 mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, complement-mediated cytotoxicity³, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)

1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
2. Vannucchi AM, et al. 2000. Blood 95:2559.
3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
4. Grisendi S, et al. 2005. Nature 437:147. (FC)
5. Bourdeau A, et al. 2007. Blood 109:4220.
6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
7. Heuser M, et al. 2007. Blood 110:1639. (FC)
8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed

Product Citations

1. Yang D, et al. 2022. Cell. 185:1905. PubMed
2. Aimaitijiang A, et al. 2022. Genes Cells. 27:25. PubMed
3. Kozuki S, et al. 2022. Genes Cells. 27:192. PubMed
4. Yousefi M, et al. 2022. Cancer Res. 82:1589. PubMed
5. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
6. Cattaneo P, et al. 2022. Nat Commun. 13:7444. PubMed
7. Wu Q, et al. 2023. Cell Rep. 42:112057. PubMed
8. Leick MB, et al. 2022. Cancer Cell. 40:494. PubMed
9. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
10. Koury D, et al. 2017. Int J Parasitol. 10.1016/j.ijpara.2017.05.009. PubMed
11. Wang L, et al. 2018. Bone Res. 6:22. PubMed
12. Ray S, et al. 2022. Elife. 11:. PubMed
13. Awida Z, et al. 2022. Int J Mol Sci. 23:. PubMed
14. Gómez-Salinero JM, et al. 2022. Nat Cardiovasc Res. 1:882. PubMed
15. Peng YJ, et al. 2023. Development. 150:. PubMed
16. Kawai H, et al. 2023. JBMR Plus. 7:e10722. PubMed
17. Kalgudde Gopal S, et al. 2023. Nat Commun. 14:3020. PubMed
18. Sucre JM, et al. 2023. JCI Insight. . PubMed
19. Menolfi D, et al. 2023. Nat Commun. 14:3618. PubMed
20. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
21. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
22. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
23. Ray S, et al. 2020. J Biol Chem. 295:7113. PubMed
24. Mourao L, et al. 2019. Sci Rep. 0.991666667. PubMed
25. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
26. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
27. Li C, et al. 2021. Mol Ther. 29:822. PubMed
28. Yanagida K, et al. 2020. Dev Cell. 779:52. PubMed
29. Larouche JA, et al. 2021. eLife. 0.416666666666667. PubMed
30. Engelbrecht E, et al. 2020. eLife. 9:e52690.. PubMed
31. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
32. Liu P, et al. 2020. Genome Res. 30:1655. PubMed
33. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
34. Yeo S, et al. 2016. Cancer Res . 76: 3397 - 3410. PubMed
35. Liu J, et al. 2012. PLoS One. 7:e44044. PubMed
36. Bilodeau M, et al. 2009. PLoS One. 4:e7500. PubMed
37. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
38. Liu S, et al. 2021. Mol Ther. 29:1918. PubMed
39. Lafkas D, et al. 2013. J Cell Biol. 203:47. PubMed
40. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
41. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
42. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
43. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
44. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
45. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
46. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
47. Negretti NM, et al. 2021. Development. 148:. PubMed
48. Shcherbina A, et al. 2020. Cell Rep. 32:107964. PubMed
49. Guihard S, et al. 2010. Blood. 115:3686. PubMed
50. Yu VWC, et al. 2017. Cell. 168:944. PubMed
51. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
52. Pal B, et al. 2017. Nat Commun.. 10.1038/s41467-017-01560-x. PubMed
53. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
54. Saleiro D, et al. 2022. Nat Commun. 13:1750. PubMed
55. Pezoldt J, et al. 2022. Nat Commun. 13:7227. PubMed
56. Nayak RR, et al. 2021. Cell Host Microbe. 29(3):362-377.e11. PubMed
57. Crowe JL, et al. 2020. Proc Natl Acad Sci U S A. 22953:117. PubMed
58. Perez E, et al. 2020. J Immunol. 205:2489. PubMed
59. Dagher T, et al. 2021. J Exp Med. 218:. PubMed
60. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
61. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
62. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
63. Shin JJ, et al. 2020. Cell Rep. 32:108093. PubMed
64. Long JT, et al. 2022. Elife. 11:. PubMed
65. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
66. Li G, et al. 2020. J Clin Invest. 130:1233. PubMed
67. Hou Y, et al. 2021. Sci Transl Med. 13:. PubMed
68. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
69. Nahrendorf W, et al. 2021. eLife. 10:00. PubMed
70. Song J, et al. 2014. Exp Cell Res. 327:123. PubMed
71. Wang H, et al. 2019. J Clin Invest. 129:598. PubMed
72. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
73. Liu W, et al. 2022. J Clin Invest. 132: . PubMed
74. Maroni G, et al. 2019. Int J Mol Sci. 20. PubMed
75. Carmody C et al. 2019. Endocrinology. 160(5):1205-1222 . PubMed
76. Fu NY, et al. 2018. Dev Cell. 47:629. PubMed
77. Siddique SM, et al. 2019. Sci Rep. 9:13977. PubMed
78. Yeo SK, et al. 2020. eLife. 9:e58810.. PubMed
79. Ma L, et al. 2022. Sci Adv. 8:eabm3470. PubMed
80. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
81. Maeda K, et al. 2021. Pathogens. 10:. PubMed
82. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
83. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
84. Cambridge E, et al. 2017. Exp Hematol. 45:64-68.e5. PubMed
85. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
86. Wang H, et al. 2020. JCI Insight. 5:00. PubMed
87. Pal B, et al. 2021. Breast Cancer Res. 23:69. PubMed
88. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
89. Khoriaty R, et al. 2014. Mol Cell Biol. 34:3721. PubMed
90. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
91. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
92. Awida Z, et al. 2021. Int J Mol Sci. 23:. PubMed
93. Yin Y, et al. 2016. J Biol Chem. 291: 6923 - 6935. PubMed
94. Rowe R, et al. 2016. J Exp Med. 213: 1497 - 1512. PubMed
95. Werner MT, et al. 2020. J Biol Chem. 295:1898. PubMed
96. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
97. Schmidts A, et al. 2020. J Immunother Cancer. 8:. PubMed
98. Murray CW, et al. 2022. Nat Commun. 13:1090. PubMed
99. Lam J, et al. 2018. Nat Commun. 9:2418. PubMed

RRID

AB_313712 (BioLegend Cat. No. 116211)
AB_313712 (BioLegend Cat. No. 116212)

Antigen Details

Structure

Associated with glycophorin A, 52 kD

Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type

Erythrocytes

Biology Area

Immunology

Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID

104231 View all products for this Gene ID

UniProt

View information about TER-119 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats