PE/Cyanine5 anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
Compare all formats See PE/Cyanine5 spectral data
Cat # Size Price Quantity Check Availability
116209 25 µg $83.00
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116210 100 µg $265.00
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References

(PubMed link indicates BioLegend citation)
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Mu Z, et al. 2022. Cell Rep. 38:110514. PubMed
  2. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  3. Zaghi M, et al. 2023. Nat Commun. 14:3212. PubMed
  4. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  5. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  6. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  7. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  8. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  9. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  10. Chen I, et al. 2016. Sci Rep. 6:27195. PubMed
  11. Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed
  12. Yankova E, et al. 2021. Nature. 593:597. PubMed
  13. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  14. Dawar S, et al. 2016. Cell Death Dis. 7:e2509. PubMed
  15. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  16. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  17. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  18. Liang G, et al. 2021. STAR Protoc. 2:100884. PubMed
  19. Dulken BW, et al. 2019. Nature. 571:205. PubMed
  20. Hohsfield LA, et al. 2020. J Neuroinflammation. 0.902083333. PubMed
  21. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  22. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  23. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  24. Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
  25. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  26. Koide S, et al. 2022. iScience. 25:103603. PubMed
  27. Li D, et al. 2020. Immunohorizons. 0.661805556. PubMed
  28. Heuser M, et al. 2009. Blood. 113:1432. PubMed
  29. Chavez JS, et al. 2022. Cells. 11:. PubMed
  30. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  31. Halvarsson C, et al. 2019. Antioxid Redox Signal. 31:211. PubMed
  32. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  33. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  34. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
RRID
AB_313710 (BioLegend Cat. No. 116209)
AB_313710 (BioLegend Cat. No. 116210)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Other Formats

View All TER-119 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse TER-119/Erythroid Cells TER-119 FC
Biotin anti-mouse TER-119/Erythroid Cells TER-119 FC
FITC anti-mouse TER-119/Erythroid Cells TER-119 FC
PE anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells TER-119 FC
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells TER-119 FC
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP anti-mouse TER-119/Erythroid Cells TER-119 FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready) TER-119 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells TER-119 FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells TER-119 FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells TER-119 PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells TER-119 FC,IHC-F,IHC-P,IP,WB,CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells TER-119 FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody TER-119 FC
Brilliant Violet 711™ anti-mouse TER-119/Erythroid Cells TER-119 FC
Spark Red™ 718 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) TER-119 FC
Spark Blue™ 574 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™) TER-119 FC
Go To Top Version: 2    Revision Date: 12/04/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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