Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody, TER-119, TER-119

Pricing & Availability

Clone: TER-119 (See other available formats)
Regulatory Status: RUO
Other Names: Ly-76
Isotype: Rat IgG2b, κ

TER-119_SparkNIR685_TER-119_Antibody_1_091721 — C57BL/6 mouse bone marrow cells were stained with anti-mouse CD45 FITC and anti-mouse TER-119 (clone TER-119) Spark NIR™ 685 (left) or anti-mouse CD45 FITC only (right).

Compare all formats See Spark NIR™ 685 spectral data

Cat #

Size

Price

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116265

25 µg

$141.00

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116266

100 µg

$293.00

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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Technical data sheet

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Day-14 fetal liver cells from a C57BL/6 mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark NIR™ 685 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark NIR™ 685 has a maximum excitation of 665 nm and a maximum emission of 685 nm.

Excitation Laser

Red Laser (633 nm)

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation¹, Western blotting¹, complement-mediated cytotoxicity³, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References (PubMed link indicates BioLegend citation)

Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
Vannucchi AM, et al. 2000. Blood 95:2559.
Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
Grisendi S, et al. 2005. Nature 437:147. (FC)
Bourdeau A, et al. 2007. Blood 109:4220.
Chappaz S, et al. 2007. Blood 110:3862. (FC)
Heuser M, et al. 2007. Blood 110:1639. (FC)
Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed

RRID

AB_2894678 (BioLegend Cat. No. 116265)
AB_2894678 (BioLegend Cat. No. 116266)

Antigen Details

Structure: Associated with glycophorin A, 52 kD
Distribution: Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
Cell Type: Erythrocytes
Biology Area: Immunology
Antigen References: 1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.
Gene ID: 104231 View all products for this Gene ID
UniProt: View information about TER-119 on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Other Formats

View All TER-119 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse TER-119/Erythroid Cells	TER-119	FC
Biotin anti-mouse TER-119/Erythroid Cells	TER-119	FC
FITC anti-mouse TER-119/Erythroid Cells	TER-119	FC
PE anti-mouse TER-119/Erythroid Cells	TER-119	FC
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Purified anti-mouse TER-119/Erythroid Cells	TER-119	FC,IHC-F,IHC-P,IP,WB
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells	TER-119	FC
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells	TER-119	FC
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells	TER-119	FC
PerCP anti-mouse TER-119/Erythroid Cells	TER-119	FC
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Pacific Blue™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready)	TER-119	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells	TER-119	PG
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells	TER-119	FC
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells	TER-119	PG
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells	TER-119	PG
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells	TER-119	FC,IHC-F,IHC-P,IP,WB,CMCD
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells	TER-119	FC
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells	TER-119	FC
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody	TER-119	FC
Brilliant Violet 711™ anti-mouse TER-119/Erythroid Cells	TER-119	FC
Spark Red™ 718 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™)	TER-119	FC
Spark Blue™ 574 anti-mouse TER-119/Erythroid Cells (Flexi-Fluor™)	TER-119	FC

Go To Top Version: 1 Revision Date: 09/17/2021

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