APC/Cyanine7 anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
MEL14_APCCy7_031010
C57BL/6 mouse splenocytes were stained with CD62L (clone MEL-14) APC/Cyanine7 (red histogram) or rat IgG2a FITC isotype control (blue histogram).
  • MEL14_APCCy7_031010
    C57BL/6 mouse splenocytes were stained with CD62L (clone MEL-14) APC/Cyanine7 (red histogram) or rat IgG2a FITC isotype control (blue histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
104427 25 µg $94.00
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104428 100 µg $248.00
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  2. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  3. Russler-Germain EV, et al. 2021. Immunity. 54:2547. PubMed
  4. Orvain C, et al. 2022. Arthritis Res Ther. 24:13. PubMed
  5. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  6. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  7. Wang K, et al. 2023. Nat Commun. 14:2950. PubMed
  8. Kurosawa M, et al. 2021. Int J Mol Sci. 22:. PubMed
  9. Stewart I et al. 2018. Immunity. 49(3):477-489 . PubMed
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  11. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  12. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  13. Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed
  14. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  15. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  16. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  17. Zhang F, et al. 2019. Nat Commun. 10:3974. PubMed
  18. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  19. Xu F, et al. 2022. Cell Death Discov. 8:142. PubMed
  20. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  21. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  22. Topham T 2010. J Immunol. 184:3841. PubMed
  23. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  24. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  25. Ly A, et al. 2020. Cell Reports. 29(8):2257-2269.e6.. PubMed
  26. Vieyra-Garcia P, et al. 2016. Clin Cancer Res. 22: 3328 - 3339. PubMed
  27. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  28. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  29. Rengarajan S, et al. 2020. Cell Rep Med. :1. PubMed
  30. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  31. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  32. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  33. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  34. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  35. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  36. Toomer K, et al. 2016. J Immunol. 196: 3665 - 3676. PubMed
  37. Webb ER, et al. 2022. iScience. 25:104995. PubMed
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  41. Pardo E, et al. 2017. PLoS One. 12(6):e0177472. PubMed
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  43. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  44. Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed
  45. Frantz PN, et al. 2021. Nat Commun. 12:6277. PubMed
  46. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  47. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  48. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  49. Ryg-Cornejo V, et al. 2016. Cell Rep. 14:68-81. PubMed
  50. Hebbandi Nanjundappa R, et al. 2017. Cell. 171:655. PubMed
  51. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
RRID
AB_830798 (BioLegend Cat. No. 104427)
AB_830798 (BioLegend Cat. No. 104428)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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