APC/Fire™ 810 anti-human CD137 (4-1BB) Antibody

Pricing & Availability
Clone
S18014C (See other available formats)
Regulatory Status
RUO
Other Names
4-1BB, ILA, CDw137, TNFRSF9
Isotype
Mouse IgG1, κ
S18014C_APC-FIRE-810_CD137_FC_071924
PHA-stimulated (3 days) human peripheral blood mononuclear cells were stained with anti-human CD3 (clone UCHT1) Brilliant Violet 421™ and anti-human CD137 (4-1BB) (clone S18014C) APC/Fire™ 810 (left) or mouse IgG1, κ APC/Fire™ 810 isotype control (right). Data shown were gated on lymphocytes.
  • S18014C_APC-FIRE-810_CD137_FC_071924
    PHA-stimulated (3 days) human peripheral blood mononuclear cells were stained with anti-human CD3 (clone UCHT1) Brilliant Violet 421™ and anti-human CD137 (4-1BB) (clone S18014C) APC/Fire™ 810 (left) or mouse IgG1, κ APC/Fire™ 810 isotype control (right). Data shown were gated on lymphocytes.
Compare all formats See APC/Fire™ 810 spectral data
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300823 25 tests $160.00
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300824 100 tests $331.00
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Description

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD137-Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Red Laser (633 nm)
RRID
AB_3662209 (BioLegend Cat. No. 300823)
AB_3662209 (BioLegend Cat. No. 300824)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 30 kD
Distribution

Activated T cells

Function
T cell costimulation
Ligand/Receptor
4-1BB ligand (CD137L)
Cell Type
Lymphocytes, NK cells, NKT cells, T cells
Biology Area
Costimulatory Molecules, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References
  1. Gruss HJ & Dower SK. 1995. Blood. 85:3378-404.
  2. Sica G & Chen L. 2000. Adv Exp Med Biol. 465:355-62.
  3. Alderson MR, et al. 1994. Eur J Immunol. 24:2219-27.
  4. Schwarz H, et al. 1996. Blood 87:2839-45.
Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org
Go To Top Version: 1    Revision Date: 07/19/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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