Spark Blue™ 574 anti-human CD137 (4-1BB) (Flexi-Fluor™) Antibody

Pricing & Availability
Clone
S18014C (See other available formats)
Regulatory Status
RUO
Other Names
4-1BB, ILA, CDw137, TNFRSF9
Isotype
Mouse IgG1, κ
Compare all formats See Spark Blue™ 574 spectral data
Cat # Size Price Quantity
285125 50 µg $394.00
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Description

CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD137-Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC
Recommended Usage

Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

RRID
AB_3662465 (BioLegend Cat. No. 285125)

Antigen Details

Structure
TNFR superfamily, type I transmembrane protein, 30 kD
Distribution

Activated T cells

Function
T cell costimulation
Ligand/Receptor
4-1BB ligand (CD137L)
Cell Type
Lymphocytes, NK cells, NKT cells, T cells
Biology Area
Costimulatory Molecules, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family
CD Molecules
Antigen References
  1. Gruss HJ & Dower SK. 1995. Blood. 85:3378-404.
  2. Sica G & Chen L. 2000. Adv Exp Med Biol. 465:355-62.
  3. Alderson MR, et al. 1994. Eur J Immunol. 24:2219-27.
  4. Schwarz H, et al. 1996. Blood 87:2839-45.
Gene ID
3604 View all products for this Gene ID
UniProt
View information about CD137 on UniProt.org
Go To Top Version: 1    Revision Date: 08/30/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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