APC/Fire™ 750 anti-mouse H-2Db Antibody

Pricing & Availability
Clone
KH95 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (BALB/c) IgG2b, κ
KH95_APCFire750_H-2Db_Antibody_1_041218
C57BL/6 mouse splenocytes were stained with H-2Db (clone KH95) APC/Fire™ 750 (filled histogram) or mouse IgG2b, κ APC/Fire™ 750 isotype control (open histogram).
  • KH95_APCFire750_H-2Db_Antibody_1_041218
    C57BL/6 mouse splenocytes were stained with H-2Db (clone KH95) APC/Fire™ 750 (filled histogram) or mouse IgG2b, κ APC/Fire™ 750 isotype control (open histogram).
  • KH95_APCFire750_H-2Db_Antibody_2_041218
    BALB/c mouse splenocytes were stained with H-2b (clone KH95) APC/Fire™ 750 (filled histogram) or mouse IgG2b, κ APC/Fire™ 750 isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Check Availability
111519 25 µg $124.00
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111520 100 µg $288.00
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Description

The KH95 antibody reacts with the H-2Db MHC class I alloantigen expressed on nucleated cells from mice of the H-2Db haplotype. H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. Reactivity with other haplotypes (e.g., a,d,f,k,n,p,q,r,s,u,v) has not been reported.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/10 mouse skin graft and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

 

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
  2. Shao H, et al. 2005. J. Immunol. 175:1851.
  3. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.
  4. Robb RJ, et al. 2012 Blood. 119:5898. PubMed
  5. Zhang P, et al. 2013. J. Immunol. 191:5291. PubMed
  6. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  7. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
  8. Hogan T, et al. 2014. PLoS Comput Biol. 10:1003805. PubMed
RRID
AB_2728133 (BioLegend Cat. No. 111519)
AB_2728133 (BioLegend Cat. No. 111520)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Db haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID
UniProt
View information about H-2Db on UniProt.org
Go To Top Version: 0    Revision Date: 04/13/2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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