Biotin anti-mouse H-2D b Antibody

Pricing & Availability
Clone
KH95 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (BALB/c) IgG2b, κ
KH95_Biotin_H-2Db_Antibody_070218_updated
C57BL/6 (left) and BALB/c (right) mouse splenocytes were stained with H-2Db Biotin (filled histogram) or mouse IgG2b, κ Biotin isotype control (open histogram) followed by Streptavidin-PE.
  • KH95_Biotin_H-2Db_Antibody_070218_updated
    C57BL/6 (left) and BALB/c (right) mouse splenocytes were stained with H-2Db Biotin (filled histogram) or mouse IgG2b, κ Biotin isotype control (open histogram) followed by Streptavidin-PE.
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Cat # Size Price Quantity Check Availability
111504 500 µg $282.00
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Description

The KH95 antibody reacts with the H-2Db MHC class I alloantigen expressed on nucleated cells from mice of the H-2Db haplotype. H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. Reactivity with other haplotypes (e.g., a,d,f,k,n,p,q,r,s,u,v) has not been reported.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/10 mouse skin graft and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
  2. Shao H, et al. 2005. J. Immunol. 175:1851.
  3. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.
  4. Robb RJ, et al. 2012 Blood. 119:5898. PubMed
  5. Zhang P, et al. 2013. J. Immunol. 191:5291. PubMed
  6. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  7. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
  8. Hogan T, et al. 2014. PLoS Comput Biol. 10:1003805. PubMed
Product Citations
  1. Ligeon LA, et al. 2016. Methods Mol Biol. 145:1519. PubMed
  2. Loi M, et al. 2019. Methods Mol Biol. 455:1880. PubMed
  3. Ponomarev E, et al. 2006. J Immunol. 176:1402. PubMed
  4. Lu SX, et al. 2021. Cell. . PubMed
RRID
AB_313509 (BioLegend Cat. No. 111504)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Db haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID
UniProt
View information about H-2Db on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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