FITC anti-mouse H-2D b Antibody

Pricing & Availability
Clone
KH95 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (BALB/c) IgG2b, κ
KH95_FITC_H-2D_Antibody_FC_1_111213
C57BL/6 mouse splenocytes were stained with H-2b (clone KH95) FITC (filled histogram) or mouse IgG2b, κ FITC isotype control (open histogram).
  • KH95_FITC_H-2D_Antibody_FC_1_111213
    C57BL/6 mouse splenocytes were stained with H-2b (clone KH95) FITC (filled histogram) or mouse IgG2b, κ FITC isotype control (open histogram).
  • KH95_FITC_H-2D_Antibody_FC_2_111213
    BALB/C mouse splenocytes were stained with H-2b (clone KH95) FITC (filled histogram), or mouse IgG2b, κ FITC isotype control (open histogram).
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability
111505 50 µg $93.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
111506 500 µg $309.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The KH95 antibody reacts with the H-2Db MHC class I alloantigen expressed on nucleated cells from mice of the H-2Db haplotype. H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. Reactivity with other haplotypes (e.g., a,d,f,k,n,p,q,r,s,u,v) has not been reported.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL/10 mouse skin graft and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-dependent cytotoxicity1, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
  2. Shao H, et al. 2005. J. Immunol. 175:1851.
  3. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.
  4. Robb RJ, et al. 2012 Blood. 119:5898. PubMed
  5. Zhang P, et al. 2013. J. Immunol. 191:5291. PubMed
  6. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
  7. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
  8. Hogan T, et al. 2014. PLoS Comput Biol. 10:1003805. PubMed
Product Citations
  1. Claser C, et al. 2019. Nat Commun. 3.361805556. PubMed
  2. Poh C, et al. 2014. Infect Immun . 82:4854. PubMed
  3. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  4. Sateriale A, et al. 2020. Cell Host & Microbe. 26(1):135-146.e5.. PubMed
  5. Nair A, et al. 2007. Brain Behav Immun. 21:791. PubMed
RRID
AB_313511 (BioLegend Cat. No. 111505)
AB_313511 (BioLegend Cat. No. 111506)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Db haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14964 View all products for this Gene ID
UniProt
View information about H-2Db on UniProt.org
Go To Top Version: 2    Revision Date: 12/05/2013

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account