Biotin anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
1_17A2_Biotin_CD3_Antibody_FC_1_072514
C57BL/6 splenocytes were stained with CD19 APC and biotinylated CD3 (clone 17A2) (top) or biotinylated rat IgG2b, κ isotype control (bottom), followed by SAV-PE.
  • 1_17A2_Biotin_CD3_Antibody_FC_1_072514
    C57BL/6 splenocytes were stained with CD19 APC and biotinylated CD3 (clone 17A2) (top) or biotinylated rat IgG2b, κ isotype control (bottom), followed by SAV-PE.
  • 2_17A2_Biotin_CD3_Antibody_FC_2_072514
  • 3_17A2_BIOTIN_CD3_Antibody_3_010620
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of biotin anti-mouse CD3 (clone 17A2), Alexa Fluor® 594 anti-mouse B220 (clone RA3-6B2) (blue) and Alexa Fluor® 488 anti-mouse CD29 (clone HMβ1-1) (green) overnight at 4°C, followed by 2.5 µg/ml of Spark YG™ 570 Streptavidin (red) for 2 hours at room temperature. The image was captured by 10X objective.
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100243 50 µg $47.00
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100244 500 µg $165.00
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  2. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  3. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  4. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  5. Lopez R, et al. 2022. Nat Biotechnol. 40:1360. PubMed
  6. Brian BF, et al. 2022. Sci Adv. 8:eabj5227. PubMed
  7. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  8. Thierry GR, et al. 2018. J Exp Med. 215:2972. PubMed
  9. Shao B, et al. 2018. Mol Med Rep. 18:920. PubMed
  10. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  11. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  12. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  13. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  14. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  15. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  16. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  17. Niss K, et al. 2020. Cell Reports. 31(11):107763. PubMed
  18. Wang Y, et al. 2021. Signal Transduct Target Ther. 6:409. PubMed
  19. Shen Z, et al. 2021. iScience. 24:103014. PubMed
  20. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  21. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  22. Dong S, et al. 2021. Nature. 591:117. PubMed
  23. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  24. Lebert-Ghali C, et al. 2016. Blood. 127: 87-90. PubMed
  25. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  26. Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
  27. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  28. Bost P, et al. 2020. Cell. 181(7):1475-1488. PubMed
  29. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  30. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  31. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  32. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  33. Zhu J et al. 2017. Cell stem cell. 20(3):374-384 . PubMed
  34. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  35. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
RRID
AB_2563946 (BioLegend Cat. No. 100243)
AB_2563946 (BioLegend Cat. No. 100244)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 2    Revision Date: 01/06/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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