Pacific Blue™ anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
17A2_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ 17A2
  • 17A2_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ 17A2
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
100213 25 µg $94.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100214 100 µg $215.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  2. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  3. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  4. Briukhovetska D, et al. 2023. Immunity. 56:143. PubMed
  5. Neff T, et al. 2012. Proc Natl Acad Sci U S A. 109:5228. PubMed
  6. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  7. Zimmerman SM, et al. 2022. Oncogene. 41:4983. PubMed
  8. Rahman SMT, et al. 2022. Cell Rep. 41:111682. PubMed
  9. Li J, et al. 2022. Elife. 11:. PubMed
  10. Reynolds MB, et al. 2023. Sci Adv. 9:eade8701. PubMed
  11. Tan J, et al. 2023. Acta Pharm Sin B. 13:632. PubMed
  12. Hamdan F, et al. 2023. Mol Ther Oncolytics. 28:264. PubMed
  13. Zhou X, et al. 2023. Sci Rep. 13:6701. PubMed
  14. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  15. Gerwing M, et al. 2020. Mol Imaging Biol. 1.959027778. PubMed
  16. Christian D, et al. 2014. Infect Immun . 82:4056. PubMed
  17. Eunju O, et al. 2014. J Virol. 88:7764. PubMed
  18. Schneider C, et al. 2018. Cell. 174:271. PubMed
  19. Lasso P, et al. 2019. Integr Cancer Ther. 18:1534735419848040. PubMed
  20. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  21. Fernandes RA, et al. 2020. eLife. 9:e58463.. PubMed
  22. van Erp EA, et al. 2019. Viruses. 0.811111111. PubMed
  23. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  24. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  25. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  26. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  27. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  28. Zhang X, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:494. PubMed
  29. Subbiah J, et al. 2022. NPJ Vaccines. 7:68. PubMed
  30. Lv H, et al. 2020. Cell Metabolism. 33(1):110-127.e5. PubMed
  31. Lalla C, et al. 2011. J Immunol. 186:4490. PubMed
  32. Wang B, et al. 2022. Nat Commun. 13:3821. PubMed
  33. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
  34. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  35. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  36. Cuburu N, et al. 2019. J Immunol. 202:1250. PubMed
  37. Divan A, et al. 2018. PLoS One. 13:e0196893. PubMed
  38. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  39. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  40. Ullrich L, et al. 2021. Front Immunol. 12:729607. PubMed
  41. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  42. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  43. Chen S, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.11. PubMed
  44. Achtman A, et al. 2009. J Leukoc Biol. 85:409. PubMed
  45. Sitrin J, et al. 2017. J Immunol. 10.4049/jimmunol.1700608. PubMed
  46. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  47. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  48. Phalke SP, et al. 2019. PLoS One. 14:e0218827. PubMed
  49. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  50. Koh KH, et al. 2019. J Clin Invest. 129:1713. PubMed
  51. Tuong ZK, et al. 2021. Cell Rep. 37:110132. PubMed
  52. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  53. Brenndörfer E, et al. 2014. J Immunol. 192:1671. PubMed
  54. Li C, et al. 2018. Cell. 174:285. PubMed
  55. Wang Z, et al. 2022. NPJ Vaccines. 7:144. PubMed
  56. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  57. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  58. Zheng Z, et al. 2021. Nat Commun. 12:6202. PubMed
  59. Liu H, et al. 2021. Vaccine. 39:7001. PubMed
  60. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  61. Baratin M, et al. 2017. Immunity. 47:349. PubMed
  62. Liu Q, et al. 2021. Adv Mater. 33:e2102852. PubMed
  63. Wang X, et al. 2018. Elife. 7:. PubMed
  64. Chiossone L, et al. 2009. Blood. 113:5488. PubMed
  65. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  66. Zhang L, et al. 2020. Cell. 442:181. PubMed
  67. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  68. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  69. Chonwerawong M, et al. 2020. Gastroenterology. 159:169. PubMed
  70. Gawish R, et al. 2022. Elife. 11:. PubMed
  71. Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed
  72. García–Rodríguez S, et al. 2018. Sci Rep. 2.664583333. PubMed
  73. Doo DW, et al. 2020. Ther Adv Med Oncol. 12:1758835920913798. PubMed
  74. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  75. Lucas ED, et al. 2020. Cell Reports. 33(2):108258. PubMed
  76. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
RRID
AB_493644 (BioLegend Cat. No. 100213)
AB_493644 (BioLegend Cat. No. 100214)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 1    Revision Date: 11/30/2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account