Biotin anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
1A8_Biotin_082808.jpg
C57BL/6 bone marrow cells stained with 1A8 biotin, followed by Sav-PE (gated on myeloid cells)
  • 1A8_Biotin_082808.jpg
    C57BL/6 bone marrow cells stained with 1A8 biotin, followed by Sav-PE (gated on myeloid cells)
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Cat # Size Price Quantity Check Availability
127603 50 µg $77.00
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127604 500 µg $270.00
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Barkaway A, et al. 2021. Immunity. . PubMed
  2. Haffner-Luntzer M, et al. 2017. Eur J Med Res. 10.1186/s40001-017-0264-y. PubMed
  3. Obi AT, et al. 2023. Sci Rep. 13:3253. PubMed
  4. Xu H, et al. 2023. Elife. 12:. PubMed
  5. Song R, et al. 2022. Immunology. 165:274. PubMed
  6. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  7. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  8. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  9. Khaw YM, et al. 2023. EMBO Rep. 24:e54228. PubMed
  10. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  11. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  12. Kim S, et al. 2020. Immunity. 53(4):759-774.e9. PubMed
  13. Blazek K, et al. 2015. J Exp Med. 212:845. PubMed
  14. Haffner–Luntzer M, et al. 2019. Proc Natl Acad Sci U S A. 116:8615. PubMed
  15. Murata A, et al. 2020. Front Immunol. 11:775. PubMed
  16. Sasse C, et al. 2022. Pathogens. 11:. PubMed
  17. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  18. Tang Y, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G156 - G165. PubMed
  19. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  20. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  21. Periasamy S, et al. 2016. PLoS Pathog. 12: 1005517. PubMed
  22. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  23. Lerman I, et al. 2019. Mol Cancer Res. 17:845. PubMed
  24. Evans R, et al. 2019. Cell Rep. 27:1967. PubMed
  25. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  26. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  27. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  28. Vaibhav K, et al. 2018. J Exp Med. 215:2636. PubMed
  29. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  30. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  31. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  32. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  33. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  34. Moorlag SJCFM, et al. 2020. Cell Rep. 33:108387. PubMed
  35. Ryzhov S, et al. 2011. J Immunol. 187:6120. PubMed
  36. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  37. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  38. Muthukrishnan G, et al. 2021. Frontiers in Immunology. 12:651515. PubMed
  39. Popova T, et al. 2016. PLoS One. 11: 0163163. PubMed
  40. Steiger S, et al. 2015. J Immunol. 195: 5495 - 5502. PubMed
  41. Yang L, et al. 2012. J Immunol. 189:3741. PubMed
  42. Chen Z, et al. 2021. Front Immunol. 12:641874. PubMed
  43. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  44. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  45. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  46. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  47. Izumi G, et al. 2021. Nat Commun. 12:5029. PubMed
  48. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  49. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  50. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  51. Mohammadpour H, et al. 2021. Cell Rep. 37:109883. PubMed
  52. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  53. Sahakian E, et al. 2017. J Leukoc Biol. 102:475. PubMed
  54. Anderson DA, et al. 2022. J Exp Med. 219:. PubMed
  55. Ubags ND, et al. 2021. J Allergy Clin Immunol. 1049:147. PubMed
  56. Bosteels C, et al. 2020. Immunity. 52(6):1039-1056.e9.. PubMed
  57. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  58. Huang X, et al. 2021. Immunity. . PubMed
  59. Schäfer AL, et al. 2021. Front Immunol. 12:696810. PubMed
  60. Morita Y, et al. 2022. Bone Res. 10:56. PubMed
  61. De Cock J, et al. 2016. Cancer Res . 76(23):6778-6784. PubMed
  62. Cai W, et al. 2019. J Neuroinflammation. 0.788194444. PubMed
  63. Jorge Domínguez‐Andrés et al. 2017. Immunity. 46(6):1059-1072 . PubMed
  64. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
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RRID
AB_1186105 (BioLegend Cat. No. 127603)
AB_1186105 (BioLegend Cat. No. 127604)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Go To Top Version: 3    Revision Date: 01/06/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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