PerCP/Cyanine5.5 anti-mouse Ly-6G Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Ly-6G transfected EL-4J cell line.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁵. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁵. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4¹⁶.

Additional reported applications (for the relevant formats) include: immunohistochemistry⁹ of frozen sections¹⁰ and paraffin-embedded sections¹¹, depletion^{4, 12-14}, and spatial biology (IBEX)^20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Zhang L, et al. 2022. Immunol Cell Biol. 100:691. PubMed
2. Kedmi R, et al. 2022. Nature. 610:737. PubMed
3. Chang CY, et al. 2022. Sci Adv. 8:eabq0615. PubMed
4. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
5. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
6. Dijkman K, et al. 2023. NPJ Vaccines. 8:66. PubMed
7. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
8. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
9. Merana GR, et al. 2022. Cell Rep. 39:110891. PubMed
10. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
11. Ostendorf BN, et al. 2022. Nature. 611:346. PubMed
12. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
13. Marritt KL, et al. 2023. Front Immunol. 13:1087991. PubMed
14. Denis M, et al. 2023. Front Immunol. 13:1011943. PubMed
15. Ryan CB, et al. 2022. Neurobiol Dis. 163:105608. PubMed
16. Fernández-García V, et al. 2022. Biomed Pharmacother. 148:112769. PubMed
17. Wong Fok Lung T, et al. 2022. Cell Metab. 34:761. PubMed
18. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
19. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
20. Cho SM et al. 2018. Journal of neurochemistry. 148(5):625-638 . PubMed
21. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
22. Ercoli G, et al. 2021. Front Immunol. 11:611661. PubMed
23. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
24. Kwon O, et al. 2016. Stem Cell Res. 16: 682-691. PubMed
25. Kim AS, et al. 2021. Cell. 184(17):4414-4429.e19. PubMed
26. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
27. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
28. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
29. Rodriguez-García A, et al. 2021. Nat Commun. 12:877. PubMed
30. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
31. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
32. Liang W, et al. 2020. Nat Commun. 4.563888889. PubMed
33. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
34. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
35. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
36. Kwon O, et al. 2014. Proc Natl Acad Sci U S A. 111:592. PubMed
37. Liu Y, et al. 2015. Sci Rep. 5:17215. PubMed
38. Fuster JJ, et al. 2020. Cell Rep. 33:108326. PubMed
39. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
40. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
41. Mukherjee D, et al. 2022. Nat Commun. 13:3747. PubMed
42. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
43. Lebratti T, et al. 2021. eLife. 10:00. PubMed
44. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
45. Privratsky JR, et al. 2018. Am J Physiol Renal Physiol. 315:F682. PubMed
46. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
47. Francian A, et al. 2021. J Drug Target. 29:754. PubMed
48. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
49. McCullough LD, et al. 2021. J Clin Invest. 131:. PubMed
50. Sugita J, et al. 2021. Nat Commun. 12:1910. PubMed
51. Klopfenstein N, et al. 2021. PLoS Pathog. 17:e1009387. PubMed
52. Moses K, et al. 2016. J Leukoc Biol. 99: 811 - 823. PubMed
53. Zhang B, et al. 2016. Mol Cell. 63: 976-89. PubMed
54. Doty DT, et al. 2020. Int J Mol Sci. 21:00. PubMed
55. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
56. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
57. Kovacic B, et al. 2014. Haematologica. 99:1006. PubMed
58. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
59. Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
60. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
61. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
62. Hildebrand KM, et al. 2021. PLoS One. 16:e0253864. PubMed
63. Zhang T, et al. 2022. Oncogene. . PubMed
64. Dang MT, et al. 2021. Cell Reports. 34(13):108917. PubMed
65. Körner A, et al. 2019. Proc Natl Acad Sci U S A. 116:20623. PubMed
66. Acker KP, et al. 2019. iScience. 19:281. PubMed
67. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
68. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
69. Zhang Y, et al. 2021. J Immunol Res. 2021:5599439. PubMed
70. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
71. Gurung P, et al. 2016. Proc Natl Acad Sci U S A. 113: 4452-4457. PubMed
72. Devaraj A, et al. 2021. J Clin Invest. 131:. PubMed
73. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
74. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
75. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
76. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
77. Shepardson K, et al. 2016. MBio. 7: e00506-16. PubMed
78. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
79. Mattila JT, et al. 2017. J Immunol. 199:806. PubMed
80. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
81. Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed
82. Dang MT, et al. 2021. STAR Protoc. 2:100957. PubMed
83. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
84. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
85. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
86. Chen Z, et al. 2016. Nat Commun. 7:11596. PubMed
87. Lokken K, et al. 2014. PLoS Pathog. 10:1004049. PubMed
88. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
89. Sun X, et al. 2020. Cell Metabolism. 31(1):189-206.e8.. PubMed
90. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
91. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
92. Chan WY, et al. 2019. Infect Immun. 87:. PubMed
93. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
94. Karki R, et al. 2020. bioRxiv. . PubMed
95. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
96. Karki R, et al. 2021. Cell. 184:149. PubMed
97. Jablonski K, et al. 2015. PLoS One. 10: 0145342. PubMed
98. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
99. Dymek B, et al. 2022. J Inflamm Res. 15:5621. PubMed
100. Milich LM, et al. 2021. J Exp Med. 218:. PubMed
101. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
102. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
103. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
104. Ireland L, et al. 2020. Front Immunol. 11:297. PubMed
105. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
106. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
107. Nakayama Y, et al. 2020. Proc Natl Acad Sci U S A. 117:14365. PubMed
108. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
109. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
110. Verma M, et al. 2021. J Exp Med. 218:. PubMed
111. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
112. Trivedi S, et al. 2020. Elife. 9:00. PubMed
113. Amber Smith, Amanda Smith 2016. Sci Rep. 6:38703. PubMed
114. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
115. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
116. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
117. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed

RRID

AB_1877271 (BioLegend Cat. No. 127615)
AB_1877271 (BioLegend Cat. No. 127616)

Antigen Details

Structure

A 21-35 kD GPI-anchorded membrane protein

Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type

Granulocytes, Macrophages, Monocytes

Biology Area

Immunology, Innate Immunity

Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID

546644 View all products for this Gene ID

UniProt

View information about Ly-6G on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats