PE/Cyanine7 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
1A8_PECy7_092409
C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7
  • 1A8_PECy7_092409
    C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability
127617 25 µg $99.00
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127618 100 µg $303.00
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Furuya Y, et al. 2014. J Virol. 88:9166. PubMed
  2. Dudek M, et al. 2015. Mucosal Immunol. . PubMed
  3. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  4. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  5. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  6. Solier S, et al. 2023. Nature. 617:386. PubMed
  7. Vereertbrugghen A, et al. 2023. J Neuroinflammation. 20:120. PubMed
  8. Wang Z, et al. 2023. Mediators Inflamm. 2023:9810733. PubMed
  9. Zhao C, et al. 2023. NPJ Regen Med. 8:29. PubMed
  10. Huang T, et al. 2023. Cell Death Dis. 14:354. PubMed
  11. Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
  12. Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed
  13. Buckland RL, et al. 2020. Curr Protoc Mouse Biol. 10:e78. PubMed
  14. Zhang Y, et al. 2022. J Cereb Blood Flow Metab. 42:952. PubMed
  15. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  16. Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed
  17. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  18. Miró L, et al. 2022. Cancers (Basel). 14: . PubMed
  19. Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed
  20. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  21. Fan R, et al. 2023. J Immunother Cancer. 11: . PubMed
  22. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  23. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  24. Kang J, et al. 2023. JCI Insight. 8: . PubMed
  25. Schick J, et al. 2023. Elife. 12: . PubMed
  26. Hu R, et al. 2023. Cell Death Discov. 9:50. PubMed
  27. Israelow B, et al. 2020. bioRxiv. . PubMed
  28. Bieren J, et al. 2015. J Immunol. 194:1154. PubMed
  29. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  30. Gawish R, et al. 2022. Elife. 11:. PubMed
  31. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  32. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  33. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  34. Buxadé M, et al. 2018. J Exp Med. 215:2901. PubMed
  35. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  36. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  37. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  38. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  39. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  40. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  41. Douglas T, et al. 2015. J Immunol. 195: 2365-2373. PubMed
  42. Mercer HL, et al. 2020. PLoS Pathog. 16:e1008572. PubMed
  43. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  44. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  45. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  46. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  47. Sanfilippo A, et al. 2015. Infect Immun . 83: 2976-2983. PubMed
  48. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  49. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  50. Jeong D, et al. 2021. eLife. 0.416666666666667. PubMed
  51. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  52. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  53. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  54. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  55. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  56. Boothby IC, et al. 2021. Nature. 599:667. PubMed
  57. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  58. Lu S, et al. 2020. JCI Insight. 5:00. PubMed
  59. Philip N, et al. 2016. PLoS Pathog. 12:e1005910. PubMed
  60. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  61. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  62. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  63. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  64. Sitnik S, et al. 2020. Mol Ther Oncolytics. 17:190. PubMed
  65. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
  66. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  67. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
  68. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  69. Donlan AN, et al. 2020. Anaerobe. 66:102275. PubMed
  70. Yu X, et al. 2022. J Extracell Vesicles. 11:e12185. PubMed
  71. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  72. Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed
  73. Singh S, et al. 2021. Cell Host Microbe. 29(3):347-361.e12. PubMed
  74. Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed
  75. Chan C, et al. 2012. Hypertension. 60:1207. PubMed
  76. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  77. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  78. Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
  79. Guo L, et al. 2021. Front Immunol. 12:785457. PubMed
  80. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  81. Galvani S, et al. 2015. Sci Signal. 8: ra79. PubMed
  82. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  83. Lasso P, et al. 2020. Front Immunol. 584959:11. PubMed
  84. Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed
  85. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  86. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  87. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  88. Nguyen GT, et al. 2021. iScience. 24(8):102871. PubMed
  89. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  90. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  91. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  92. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  93. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  94. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  95. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
  96. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  97. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  98. Kaufmann E et al. 2018. Cell. 172(1-2):176-190 . PubMed
  99. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  100. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  101. Yang Y, et al. 2022. Elife. 11:. PubMed
  102. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  103. Lei J, et al. 2021. Theranostics. 4251:11. PubMed
  104. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  105. Bryant J, et al. 2014. J Immunol. 192:6092. PubMed
  106. Fischer KJ, et al. 2019. J Immunol. 202:2027. PubMed
  107. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  108. Poczobutt JM, et al. 2021. Am J Respir Cell Mol Biol. 64:629. PubMed
  109. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  110. Almishri W, et al. 2015. J Hepatol. 63: 943-951. PubMed
  111. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  112. Bravo-Caparrós I, et al. 2020. FASEB J. 34:5951. PubMed
  113. Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed
  114. Chen F, et al. 2016. J Immunol. 196: 4390 - 4399. PubMed
  115. Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed
  116. Johansson J, et al. 2013. J Neurosci. 33:16016. PubMed
  117. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  118. Ngamsri KC, et al. 2020. Front Immunol. 11:407. PubMed
  119. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  120. Upadhya R, et al. 2016. MBio. 7: 00547-16. PubMed
  121. Alexander K, et al. 2016. Immunol Cell Biol. 95:7-16. PubMed
  122. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  123. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  124. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  125. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  126. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  127. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  128. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  129. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  130. Wang X, et al. 2021. Cell. 184:5357. PubMed
  131. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  132. Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed
  133. Ling Y, et al. 2016. Pharmacol Res. S1043-6618(16)31329-9. PubMed
  134. Nguyen GT, et al. 2020. eLife. 9:e56656.. PubMed
  135. Tang W, et al. 2021. Front Cell Dev Biol. 9:728057. PubMed
  136. Tang S, et al. 2022. Virol J. 19:32. PubMed
  137. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  138. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  139. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  140. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  141. Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed
  142. Braza MS et al. 2018. Immunity. 49(5):819-828 . PubMed
  143. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  144. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  145. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  146. Wang X, et al. 2021. Theranostics. 11:209. PubMed
  147. Lee J, et al. 2015. Nat Commun. 6: 8499. PubMed
  148. Goc J, et al. 2021. Cell. 184:5015. PubMed
  149. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  150. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  151. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
  152. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  153. Dietschmann A, et al. 2020. Eur J Immunol. 50:1044. PubMed
  154. Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed
  155. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  156. Wu L, et al. 2021. Cell Death Dis. 12:1064. PubMed
RRID
AB_1877261 (BioLegend Cat. No. 127617)
AB_1877261 (BioLegend Cat. No. 127618)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Go To Top Version: 3    Revision Date: 01/06/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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