Brilliant Violet 605™ anti-mouse CD274 (B7-H1, PD-L1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence⁴, blocking^6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections^{4, 11}. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)

1. Maier H, et al. 2007. J. Immunol. 178:2714.
2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
4. Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC)
5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
12. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed

Product Citations

1. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
2. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
3. Potluri HK, et al. 2022. J Immunother Cancer. 10:. PubMed
4. Willingham SB, et al. 2018. Cancer Immunol Res. 6:1136. PubMed
5. Wu S, et al. 2021. Nat Cancer. 2:189. PubMed
6. Taylor D, et al. 2022. Front Immunol. 13:936129. PubMed
7. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
8. Bryant AJ, et al. 2018. Pulm Circ. 9:2045894018823520. PubMed
9. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
10. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
11. Lin J, et al. 2021. Cancer Immunol Res. 9:1413. PubMed
12. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed

RRID

AB_2563635 (BioLegend Cat. No. 124321)

Antigen Details

Structure

40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily

Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Ligand/Receptor

PD-1 (PDCD1)

Cell Type

B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells

Biology Area

Cancer Biomarkers, Costimulatory Molecules, Immunology

Molecular Family

Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors

Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.

Gene ID

60533 View all products for this Gene ID

UniProt

View information about CD274 on UniProt.org

Other Formats