Ultra-LEAF™ Purified anti-mouse CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
10F.9G2 (See other available formats)
Regulatory Status
RUO
Other Names
B7-H1, PD-L1
Isotype
Rat IgG2b, κ
10fdot9G2_LEAF_030209
C57/B6 mouse splenocytes were stained with Ultra-LEAF™ purified anti-CD274 (clone 10F.9G2) (red line) or purified rat IgG2b, κ isotype control (green line), followed by biotinylated anti-rat IgG and Sav-PE.
  • 10fdot9G2_LEAF_030209
    C57/B6 mouse splenocytes were stained with Ultra-LEAF™ purified anti-CD274 (clone 10F.9G2) (red line) or purified rat IgG2b, κ isotype control (green line), followed by biotinylated anti-rat IgG and Sav-PE.
Compare all formats
Cat # Size Price Quantity Check Availability
124337 100 µg $110.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
124318 1 mg $149.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
124338 5 mg $539.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
124339 25 mg $1408.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
124340 50 mg $2354.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
124341 100 mg $3383.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote

Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume or 100 µL of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Maier H, et al. 2007. J. Immunol. 178:2714.
  2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
  3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
  4. Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC)
  5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
  6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
  7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
  8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
  9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
  10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
  12. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
Product Citations
  1. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  2. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed
  3. Zhang Y, et al. 2023. J Immunol Res. 2023:5867047. PubMed
  4. Stieg DC, et al. 2023. Cancer Res Commun. 1200:3. PubMed
  5. Muthumani K, et al. 2012. J Immunol. 187:2932. PubMed
  6. Dagkonaki A, et al. 2022. Front Immunol. 13:972003. PubMed
  7. Bonamichi-Santos R, et al. 2021. Front Immunol. 12:655958. PubMed
  8. Cripps J, et al. 2010. Hepatology. 52:1350. PubMed
RRID
AB_2800597 (BioLegend Cat. No. 124337)
AB_2800597 (BioLegend Cat. No. 124318)
AB_2800597 (BioLegend Cat. No. 124338)
AB_2800597 (BioLegend Cat. No. 124339)
AB_2800597 (BioLegend Cat. No. 124340)
AB_2800597 (BioLegend Cat. No. 124341)

Antigen Details

Structure
40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.

Gene ID
60533 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org
Go To Top Version: 4    Revision Date: 01/08/2021

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account