PE/Dazzle™ 594 anti-mouse CD274 (B7-H1, PD-L1) Antibody

Pricing & Availability
Clone
10F.9G2 (See other available formats)
Regulatory Status
RUO
Other Names
B7-H1, PD-L1
Isotype
Rat IgG2b, κ
10F.9G2_PEDZL_CD274_Antibody_FC_042915
C57BL/6 mouse splenocytes were stained with CD274 (clone 10F.9G2) PE/Dazzle™ 594 (filled histogram) and rat IgG2b, κ PE/Dazzle™ 594 (open histogram).
  • 10F.9G2_PEDZL_CD274_Antibody_FC_042915
    C57BL/6 mouse splenocytes were stained with CD274 (clone 10F.9G2) PE/Dazzle™ 594 (filled histogram) and rat IgG2b, κ PE/Dazzle™ 594 (open histogram).
Compare all formats See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability
124323 25 µg $141.00
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124324 100 µg $347.00
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Description

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes. B7-H1 is one of the ligands of PD-1. The interaction of B7-H1 with PD-1 plays an important role in the inhibition of T cell responses. Other studies have shown that B7-H1 is able to costimulate T cell growth and cytokine production. CD274 is involved in costimulation essential for T cell proliferation and production of IL-10 and IFN-γ, in an IL-2-dependent and a PD-1-independent manner. Its interaction with PD-1 inhibits T cell proliferation and cytokine production.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence4, blocking6,7,8,9, and immunohistochemistry of acetone-fixed frozen sections4, 11. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 124303). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 124318) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Maier H, et al. 2007. J. Immunol. 178:2714.
  2. Meng Q, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:4444. PubMed
  3. Scarlett UK, et al. 2012. J Exp Med. 209:495. PubMed
  4. Grabie N, et al. 2007. Circulation 116:2062. (IF, IHC)
  5. Paterson AM, et al. 2011. J. Immunol. 187:1097.
  6. Channappanavar R, et al. 2012. PLoS One 7:e39757. (Block)
  7. Schreiber HA, et al. 2010. PLoS One 5:e11453. (Block) PubMed
  8. Muthumani K, et al. 2011. J. Immunol. 187:2932. (Block) PubMed
  9. Cripps JG, et al. 2010. Hepatology 52:1350. (Block) PubMed
  10. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  11. Riella LV, et al. 2011. Am. J. Transplant 11:832-40. (IHC)
  12. Lei GS, et al. 2015. Infect Immun. 83:572. PubMed
Product Citations
  1. Campisi L, et al. 2022. Nature. 606:945. PubMed
  2. Molina MS, et al. 2022. PLoS One. 17:e0273075. PubMed
  3. Al-Saafeen BH, et al. 2023. Front Immunol. 13:1017780. PubMed
  4. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
  5. Liu N, et al. 2023. Cell Death Dis. 14:170. PubMed
  6. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  7. Nadafi R, et al. 2020. Cell Reports. 30(12):4110-4123. PubMed
  8. Shiao SL, et al. 2021. Cancer Cell. 39:1202. PubMed
  9. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  10. Ryan NM, et al. 2022. Front Immunol. 13:932742. PubMed
  11. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  12. Lau P, et al. 2022. Cell Mol Immunol. :. PubMed
  13. Chen KE, et al. 2021. J Neuroinflammation. 18:140. PubMed
  14. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  15. Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
RRID
AB_2565638 (BioLegend Cat. No. 124323)
AB_2565638 (BioLegend Cat. No. 124324)

Antigen Details

Structure
40 kD type I transmembrane protein member of B7 family within the immunoglobulin receptor superfamily
Distribution

T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes

Ligand/Receptor
PD-1 (PDCD1)
Cell Type
B cells, Dendritic cells, Endothelial cells, Monocytes, NK cells, T cells
Biology Area
Cancer Biomarkers, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sharpe A, et al. 2007. Nat. Immunol. 8:239.
2. Dong H, et al. 1999. Nat. Med. 5:1365.
3. Freeman G, et al. 2000. J. Exp. Med. 192:1027.

Gene ID
60533 View all products for this Gene ID
UniProt
View information about CD274 on UniProt.org
Go To Top Version: 1    Revision Date: 04/30/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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