Brilliant Violet 605™ anti-mouse CD62L Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C3H/eb mouse B lymphoma 38C-13

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.

Concentration

µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation^1-3, complement-dependent cytotoxicity⁴, in vivo and in vitro blocking of adhesion^1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections⁶. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)

1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
7. Olver S, et al. 2006. Cancer Res. 66:571.
8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
11. Lee JW, et al. 2006. Nature Immunol. 8:181.
12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed

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RRID

AB_2563058 (BioLegend Cat. No. 104437)
AB_2563058 (BioLegend Cat. No. 104438)

Antigen Details

Structure

Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)

Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function

Lymphocyte homing to HEV, rolling on activated endothelium

Ligand/Receptor

CD34, GlyCAM-1, MAdCAM-1

Cell Type

B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs

Biology Area

Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID

20343 View all products for this Gene ID

UniProt

View information about CD62L on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats