Brilliant Violet 650™ anti-mouse CD117 (c-kit) Antibody, CD117, 2B8

Pricing & Availability

Clone: 2B8 (See other available formats)
Regulatory Status: RUO
Other Names: c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype: Rat IgG2b, κ

2B8_BV650_CD117_Antibody_072320 — C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (c-kit) (clone 2B8) Brilliant Violet 650™ (left) or rat IgG2b, κ Brilliant Violet 650™ isotype control (right).

Compare all formats See Brilliant Violet 650™ spectral data

Cat #

Size

Price

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105853

50 µg

$273.00

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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Technical data sheet

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse bone marrow mast cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, immunohistochemistry of acetone fixed frozen sections², and spatial biology (IBEX)^5,6. The 2B8 antibody does not block c-Kit activity.

Application References (PubMed link indicates BioLegend citation)

Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

RRID

AB_2876414 (BioLegend Cat. No. 105853)

Antigen Details

Structure: Ig superfamily, 145 kD
Distribution: Hematopoietic stem cells, AML, mast cells
Function: Growth factor receptor, tyrosine kinase
Ligand/Receptor: Stem Cell Factor (SCF)
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.
Gene ID: 16590 View all products for this Gene ID
UniProt: View information about CD117 on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Other Formats

View All CD117 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD117 (c-Kit)	2B8	FC
Biotin anti-mouse CD117 (c-Kit)	2B8	FC
FITC anti-mouse CD117 (c-Kit)	2B8	FC
PE anti-mouse CD117 (c-Kit)	2B8	FC
PE/Cyanine5 anti-mouse CD117 (c-Kit)	2B8	FC
Purified anti-mouse CD117 (c-Kit)	2B8	FC,IHC-F
PE/Cyanine7 anti-mouse CD117 (c-Kit)	2B8	FC
Alexa Fluor® 488 anti-mouse CD117 (c-Kit)	2B8	FC,IHC-F
Alexa Fluor® 647 anti-mouse CD117 (c-Kit)	2B8	FC,IHC-F
Pacific Blue™ anti-mouse CD117 (c-Kit)	2B8	FC
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit)	2B8	FC
PerCP anti-mouse CD117 (c-kit)	2B8	FC
APC/Cyanine7 anti-mouse CD117 (c-kit)	2B8	FC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit)	2B8	FC,SB
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready)	2B8	FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit)	2B8	FC
Brilliant Violet 711™ anti-mouse CD117 (c-Kit)	2B8	FC
Alexa Fluor® 594 anti-mouse CD117 (c-Kit)	2B8	IHC-F,3D IHC
APC/Fire™ 750 anti-mouse CD117 (c-Kit)	2B8	FC
Brilliant Violet 510™ anti-mouse CD117 (c-Kit)	2B8	FC
Brilliant Violet 785™ anti-mouse CD117 (c-Kit)	2B8	FC
TotalSeq™-A0012 anti-mouse CD117 (c-kit)	2B8	PG
Brilliant Violet 605™ anti-mouse CD117 (c-Kit)	2B8	FC
Alexa Fluor® 700 anti-mouse CD117 (c-Kit)	2B8	FC
TotalSeq™-B0012 anti-mouse CD117 (c-Kit)	2B8	PG
TotalSeq™-C0012 anti-mouse CD117 (c-Kit)	2B8	PG
Spark NIR™ 685 anti-mouse CD117 (c-kit)	2B8	FC
Brilliant Violet 650™ anti-mouse CD117 (c-kit)	2B8	FC
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™)	2B8	FC
Spark Blue™ 574 anti-mouse CD117 (c-kit) (Flexi-Fluor™)	2B8	FC
Spark Blue™ 550 anti-mouse CD117 (c-kit) (Flexi-Fluor™)	2B8	FC

Go To Top Version: 1 Revision Date: 07/23/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.