FITC anti-mouse CD11a Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

C57BL/6 mouse splenic secondary cytotoxic T cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The M17/4 antibody can block CD11a-mediated cellular adhesion. Additional reported applications of this antibody (for the relevant formats) include: immunoprecipitation^1,2, in vitro blocking of cell-cell adhesion^1,2 and FOXP3 expression⁵, and immunohistochemical staining of acetone-fixed frozen sections³. The M17/4 antibody does not block the binding of 2D7 antibody (Cat. No. 101002) to CD11a. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101110). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 101118) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)

1. Sanchez-Madrid F, et al. 1982. Cell Immunol. 73:1. (IP, Block)
2. Kuhlman P, et al. 1991. J. Immunol. 146:1773. (IP, Block)
3. Mizgerd JP, et al. 1997. J. Exp. Med. 186:1357. (IHC)
4. Hailman E and Allen PM. 2005. J. Immunol. 175:4847. (FC)
5. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (Block) PubMed

Product Citations

1. Gültner S, et al. 2010. Eur J Immunol. 40:3403. PubMed
2. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
3. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
4. Halford W, et al. 2015. PLoS One. 10: 0145228. PubMed
5. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
6. Gurung P, et al. 2011. J Immunol. 187:2148. PubMed
7. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
8. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
9. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
10. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
11. Martin MD, et al. 2020. Cell Rep. 31:107508. PubMed
12. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
13. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
14. Kumar R, et al. 2020. Cell Reports. 30(8):2512-2525. PubMed

RRID

AB_312779 (BioLegend Cat. No. 101106)

Antigen Details

Structure

Integrin family, associates with integrin β2 (CD18), 180 kD

Distribution

Lymphocytes, granulocytes, monocytes, macrophages

Function

Cellular adhesion

Ligand/Receptor

ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50)

Cell Type

Granulocytes, Lymphocytes, Macrophages, Monocytes, Tregs

Biology Area

Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience

Molecular Family

Adhesion Molecules, CD Molecules

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lub M, et al. 1995. Immunol. Today 16:479.

Gene ID

16408 View all products for this Gene ID

UniProt

View information about CD11a on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats