HRP Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Product Details

Verified Reactivity

Rabbit

Antibody Type

Polyclonal

Host Species

Donkey

Formulation

Aqueous buffered solution containing a carrier protein and preservatives.

Preparation

The polyclonal antibody was purified from donkey antiserum by rabbit immunoglobulin affinity chromatography.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

To obtain lot-specific expiration date, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product

Application

WB - Quality tested
ELISA, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to detemine optimal conditions. Avoid using diluent solutions containg sodium azide. Sodium azide is an inhibitor of horseradish peroxidase. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations

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RRID

AB_2099368 (BioLegend Cat. No. 406401)

Antigen Details

Gene ID

100343867 View all products for this Gene ID

UniProt

View information about Rabbit IgG on UniProt.org

Related Protocols

• Western Blotting Protocol

Other Formats