PE Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
Poly4064_PE_Asialo-GM1_Antibody_FC_042414.jpg
C57BL/6 splenocytes were stained with NK-1.1 APC and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) PE (top image).
  • Poly4064_PE_Asialo-GM1_Antibody_FC_042414.jpg
    C57BL/6 splenocytes were stained with NK-1.1 APC and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) PE (top image).
Compare all formats See PE spectral data
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406421 100 µg $104.00
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Technical data sheet

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
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RRID
AB_2563484 (BioLegend Cat. No. 406421)
Go To Top Version: 2    Revision Date: 02/09/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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