Pacific Blue™ anti-human IgM Antibody

Pricing & Availability
Clone
MHM-88 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin M
Isotype
Mouse IgG1, κ
MHM-88_PB_1_060710
Over night cultured human peripheral blood lymphocytes stained with CD19 (HIB19) PE and MHM-88 Pacific Blue™ (top) or mouse IgG1, κ Pacific Blue™ isotype control (bottom)
  • MHM-88_PB_1_060710
    Over night cultured human peripheral blood lymphocytes stained with CD19 (HIB19) PE and MHM-88 Pacific Blue™ (top) or mouse IgG1, κ Pacific Blue™ isotype control (bottom)
  • MHM-88_PB_2_060710
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability
314513 25 µg $165.00
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314514 100 µg $347.00
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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed
Product Citations
  1. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  2. Ghraichy M, et al. 2021. Elife. 10:. PubMed
  3. Hubbard N, et al. 2016. Blood. 127: 2513 - 2522. PubMed
  4. Catera R, et al. 2017. Mol Med. 23:1. PubMed
  5. Levy Y, et al. 2021. iScience. 24:102711. PubMed
  6. Rouers A, et al. 2021. iScience. 24:102482. PubMed
  7. Wiedemann A, et al. 2020. Nat Commun. 3.048611111. PubMed
RRID
AB_10574306 (BioLegend Cat. No. 314513)
AB_10574306 (BioLegend Cat. No. 314514)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type
B cells
Biology Area
Immunology
Gene ID
3507 View all products for this Gene ID
UniProt
View information about IgM on UniProt.org

Other Formats

View All IgM Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human IgM MHM-88 FC,ELISA
PE anti-human IgM MHM-88 FC
Biotin anti-human IgM MHM-88 FC,ELISA
FITC anti-human IgM MHM-88 FC
APC anti-human IgM MHM-88 FC
PerCP/Cyanine5.5 anti-human IgM MHM-88 FC
Pacific Blue™ anti-human IgM MHM-88 FC
Brilliant Violet 421™ anti-human IgM MHM-88 FC
APC/Cyanine7 anti-human IgM MHM-88 FC
Brilliant Violet 570™ anti-human IgM MHM-88 FC
Brilliant Violet 510™ anti-human IgM MHM-88 FC
Brilliant Violet 605™ anti-human IgM MHM-88 FC
Brilliant Violet 650™ anti-human IgM MHM-88 FC
Purified anti-human IgM (Maxpar® Ready) MHM-88 FC,CyTOF®
PE/Dazzle™ 594 anti-human IgM MHM-88 FC
PE/Cyanine7 anti-human IgM MHM-88 FC
Alexa Fluor® 488 anti-human IgM MHM-88 FC
Alexa Fluor® 647 anti-human IgM MHM-88 FC
Alexa Fluor® 700 anti-human IgM MHM-88 FC
Brilliant Violet 711™ anti-human IgM MHM-88 FC
TotalSeq™-A0136 anti-human IgM MHM-88 PG
TotalSeq™-C0136 anti-human IgM MHM-88 PG
Brilliant Violet 785™ anti-human IgM MHM-88 FC
APC/Fire™ 750 anti-human IgM MHM-88 FC
TotalSeq™-B0136 anti-human IgM MHM-88 PG
Spark Violet™ 423 anti-human IgM Antibody MHM-88 FC
TotalSeq™-D0136 anti-human IgM MHM-88 PG
Spark Blue™ 550 anti-human IgM MHM-88 FC
Spark Blue™ 515 anti-human IgM MHM-88 FC
PE/Fire™ 700 anti-human IgM MHM-88 FC
Spark YG™ 593 anti-human IgM MHM-88 FC
PE/Fire™ 640 anti-human IgM MHM-88 FC
Spark Red™ 718 anti-human IgM (Flexi-Fluor™) MHM-88 FC
APC anti-human IgM MHM-88 FC
PE anti-human IgM MHM-88 FC
Brilliant Violet 750™ anti-human IgM MHM-88 FC
Go To Top Version: 3    Revision Date: 10/10/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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