- Clone
- 1C1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-3R common β chain, CDw131
- Isotype
- Mouse IgG1, κ
-
CDw131 transfected cells stained with purified 1C1 and then detected with anti-mouse IgGs FITC
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 306104 | 100 tests | $219.00 | |||
CD131, also known as the IL-3R common β subunit (βC), is a 95-120 kD type I transmembrane glycoprotein and belongs to the Ig superfamily. The common β subunit associates with the specific α subunits of IL-3 receptor, IL-5 receptor and GM-CSF receptor to form high affinity receptors for these cytokines. These cytokine receptors are expressed by neutrophils, eosinophils, monocytes, endothelial cells, fibroblasts and hematopoietic progenitor cells and play a crucial role in growth/activation of eosinophils and in the inflammatory response. The 1C1 antibody is a non-blocking antibody.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IL-3R β chain transfected COS cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting1,2 and immunoprecipitation2. The 1C1 antibody does not block binding of IL-3 and is a non-neutralizing antibody.
-
Application References
(PubMed link indicates BioLegend citation) -
- Stomski F, et al. 1998. J. Biol. Chem. 273:1192. (WB)
- Stomski F, et al. 1999. Blood 94:1933. (IP WB)
- Mark A, et al. 2000. Mol. Cell 6:99.
- Product Citations
-
- RRID
-
AB_2085808 (BioLegend Cat. No. 306104)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, associates with the α subunit of IL-3, IL-5, GM-CSF receptors, 95-120 kD
- Distribution
-
Low levels on monocytes, granulocytes, eosinophils, basophils, hematopoietic progenitors, endothelial cells
- Function
- Signal transducing molecule of IL-3, IL-5, GM-CSF receptors
- Ligand/Receptor
- IL-3, IL-5, GM-CSF
- Cell Type
- Basophils, Endothelial cells, Eosinophils, Granulocytes, Hematopoietic stem and progenitors, Monocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Sun Q, et al. 1999. Blood 94:1943.
2. Woodcock J, et al. 1997. Blood 90:3005.
3. Lopez A, et al. 1991. J. Biol. Chem. 266:24741. - Gene ID
- 1439 View all products for this Gene ID
- UniProt
- View information about CD131 on UniProt.org
Other Formats
View All CD131 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD131 | 1C1 | FC |
| Purified anti-human CD131 | 1C1 | FC,IP,WB |
| TotalSeq™-A0931 anti-human CD131 | 1C1 | PG |
| TotalSeq™-B0931 anti-human CD131 | 1C1 | PG |
| TotalSeq™-C0931 anti-human CD131 | 1C1 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD131
CDw131 transfected cells stained with purified 1C1 and then ... -
Purified anti-human CD131
CD131 transfected cell stained with purified 1C1, followed b... -
TotalSeq™-A0931 anti-human CD131
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TotalSeq™-B0931 anti-human CD131
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TotalSeq™-C0931 anti-human CD131