- Clone
- 1C1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-3R common β chain, CDw131
- Isotype
- Mouse IgG1, κ
- Barcode Sequence
- CTGCATGAGACCAAA
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 306109 | 10 µg | $369.00 | |||
CD131, also known as the IL-3R common β subunit (βC), is a 95-120 kD type I transmembrane glycoprotein and belongs to the Ig superfamily. The common β subunit associates with the specific α subunits of IL-3 receptor, IL-5 receptor and GM-CSF receptor to form high affinity receptors for these cytokines. These cytokine receptors are expressed by neutrophils, eosinophils, monocytes, endothelial cells, fibroblasts and hematopoietic progenitor cells and play a crucial role in growth/activation of eosinophils and in the inflammatory response. The 1C1 antibody is a non-blocking antibody.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human IL-3R β chain transfected COS cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting1,2 and immunoprecipitation2. The 1C1 antibody does not block binding of IL-3 and is a non-neutralizing antibody.
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) -
- Stomski F, et al. 1998. J. Biol. Chem. 273:1192. (WB)
- Stomski F, et al. 1999. Blood 94:1933. (IP WB)
- Mark A, et al. 2000. Mol. Cell 6:99.
- RRID
-
AB_3662396 (BioLegend Cat. No. 306109)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, associates with the α subunit of IL-3, IL-5, GM-CSF receptors, 95-120 kD
- Distribution
-
Low levels on monocytes, granulocytes, eosinophils, basophils, hematopoietic progenitors, endothelial cells
- Function
- Signal transducing molecule of IL-3, IL-5, GM-CSF receptors
- Ligand/Receptor
- IL-3, IL-5, GM-CSF
- Cell Type
- Basophils, Endothelial cells, Eosinophils, Granulocytes, Hematopoietic stem and progenitors, Monocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Sun Q, et al. 1999. Blood 94:1943.
2. Woodcock J, et al. 1997. Blood 90:3005.
3. Lopez A, et al. 1991. J. Biol. Chem. 266:24741. - Gene ID
- 1439 View all products for this Gene ID
- UniProt
- View information about CD131 on UniProt.org
Other Formats
View All CD131 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD131 | 1C1 | FC |
| Purified anti-human CD131 | 1C1 | FC,IP,WB |
| TotalSeq™-A0931 anti-human CD131 | 1C1 | PG |
| TotalSeq™-B0931 anti-human CD131 | 1C1 | PG |
| TotalSeq™-C0931 anti-human CD131 | 1C1 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD131
CDw131 transfected cells stained with purified 1C1 and then ... -
Purified anti-human CD131
CD131 transfected cell stained with purified 1C1, followed b... -
TotalSeq™-A0931 anti-human CD131
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TotalSeq™-B0931 anti-human CD131
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TotalSeq™-C0931 anti-human CD131