PE anti-mouse CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A7R34 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2a, κ
A_A7R34_PE_100809
C57BL/6 mouse splenocytes stained with rat IgG2a (RTK2758) PE isotype control and CD3 (145-2C11) APC
  • A_A7R34_PE_100809
    C57BL/6 mouse splenocytes stained with rat IgG2a (RTK2758) PE isotype control and CD3 (145-2C11) APC
  • B_A7R34_PE_100809
    C57BL/6 mouse splenocytes stained with A7R34 PE and CD3 (145-2C11) APC
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Cat # Size Price Quantity Check Availability
135009 50 µg $110.00
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135010 200 µg $365.00
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-7Ra-IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

A7R34 is able to block clone SB/199 binding to IL-7R.

Application References

(PubMed link indicates BioLegend citation)
  1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
  2. Hashi H, et al. 2001. J. Immunol. 166:3702.
  3. Taylor R, et al. 2007. J. Immunol. 178:5659.
  4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
  5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations
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  5. Campisi L, et al. 2022. Nature. 606:945. PubMed
  6. Lin YH 2023. Immunity. 56(1):207-223.e8. PubMed
  7. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  8. Zheng X, et al. 2019. PLoS Pathog. 15:e1008036. PubMed
  9. Fan NW, et al. 2023. FASEB J. 37:e22855. PubMed
  10. Wei H, et al. 2021. Malar J. 20:89. PubMed
  11. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  12. Wang D, et al. 2018. Immunity. 48:659. PubMed
  13. Gupta SS, et al. 2019. Cell Rep. 29:1862. PubMed
  14. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  15. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  16. Parriott G, et al. 2020. Immunology. 160:280. PubMed
  17. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  18. Zebley CC, et al. 2021. Cell Rep. 37:109796. PubMed
  19. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  20. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  21. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  22. Flommersfeld S, et al. 2021. Immunity. :. PubMed
  23. Prosser A, et al. 2021. Cell Reports. 35(7):109141. PubMed
  24. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  25. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  26. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  27. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  28. de Lucía Finkel P, et al. 2021. Sci Rep. 11:12233. PubMed
  29. La Rose AM, et al. 2021. Mol Metab. 53:101265. PubMed
  30. Belyaev N, et al. 2010. Nat Immunol. 11:477. PubMed
  31. White C, et al. 2015. J Immunol. 194:697. PubMed
  32. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  33. Chen H, et al. 2021. iScience. 24:103387. PubMed
  34. Pandit M, et al. 2021. Eur J Immunol. 51:1461. PubMed
  35. Dekhtiarenko I, et al. 2016. PLoS Pathog. 12:e1006072. PubMed
  36. Chang P, et al. 2012. J Immunol. 189: 701. PubMed
  37. Wang D, et al. 2022. EMBO Rep. 23:e53691. PubMed
  38. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  39. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  40. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
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  43. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  44. Prosser A, et al. 2021. STAR Protoc. 2:100810. PubMed
  45. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
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RRID
AB_1937251 (BioLegend Cat. No. 135009)
AB_1937251 (BioLegend Cat. No. 135010)

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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