PE/Cyanine5 anti-mouse CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A7R34 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Rat IgG2a, κ
A7R34_PECyanine5_CD127_Antibody_1_022624
C57BL/6 mouse splenocytes stained with CD3 (clone 145-2C11) FITC and CD127 (clone A7R34) PE/Cyanine5 (left) or rat IgG2a, κ isotype control (right).
  • A7R34_PECyanine5_CD127_Antibody_1_022624
    C57BL/6 mouse splenocytes stained with CD3 (clone 145-2C11) FITC and CD127 (clone A7R34) PE/Cyanine5 (left) or rat IgG2a, κ isotype control (right).
Compare all formats See PE/Cyanine5 spectral data
Cat # Size Price Quantity Check Availability
135015 50 µg $147.00
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135016 200 µg $387.00
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be an useful marker for identifying memory and effector T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cells proliferation and development.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-7Ra-IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.15 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

A7R34 is able to block clone SB/199 binding to IL-7R.

Application References

(PubMed link indicates BioLegend citation)
  1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
  2. Hashi H, et al. 2001. J. Immunol. 166:3702.
  3. Taylor R, et al. 2007. J. Immunol. 178:5659.
  4. Mazzon C, et al. 2011. Blood. 118:2733. PubMed
  5. Jin J, et al. 2011. J. Immunol. doi:10.4049/jimmunol.1001238. PubMed
Product Citations
  1. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  2. Moudra A, et al. 2021. J Immunol. 206:2109. PubMed
  3. Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
  4. Peng X, et al. 2014. J Immunol. 193:1268. PubMed
  5. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  6. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  7. Green CD et al. 2018. Developmental cell. 46(5):651-667 . PubMed
  8. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  9. Toubal A, et al. 2020. Nat Commun. 3755:11. PubMed
  10. Cai Z, et al. 2020. Cell Rep. 31:107816. PubMed
  11. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  12. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  13. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
RRID
AB_1937261 (BioLegend Cat. No. 135015)
AB_1937261 (BioLegend Cat. No. 135016)

Antigen Details

Structure
Type I transmembrane glycoprotein, associate with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. Okuno Y, et al. 2001. P. Natl. Acad. Sci. USA 99:6246.
3. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.

Gene ID
16197 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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