PE/Fire™ 640 anti-human CD3 Antibody, CD3, SK7

Pricing & Availability

Clone: SK7 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T3, CD3ε
Isotype: Mouse IgG1, κ

Human peripheral blood lymphocytes were stained with CD3 (clone SK7) PE/Fire™ 640 (filled histogram), or unstained control (open histogram).

Compare all formats See PE/Fire™ 640 spectral data

Cat #

Size

Price

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344859

25 tests

$136.00

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344860

100 tests

$282.00

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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Technical data sheet

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections^4,5,8, immunofluorescent staining⁶, and Western blotting³.

Application References (PubMed link indicates BioLegend citation)

Kan EA, et al. 1983. J. Immunol. 131:536.
Wood GS, et al. 1985. Am. J. Pathol. 120:371.
Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
Carbone A, et al. 1999. Blood 93:2319. (IHC)
Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
Rutjens E, et al. 2007. J. Immunol. 178:1702.
Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)

RRID

AB_2860896 (BioLegend Cat. No. 344859)
AB_2860896 (BioLegend Cat. No. 344860)

Antigen Details

Structure: Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution: Mature T and NK T cells, during thymocyte differentiation
Function: Antigen recognition, signal transduction, T cell activation
Ligand/Receptor: Peptide antigen bound to MHC
Cell Type: NKT cells, T cells, Tregs
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, TCRs
Antigen References: 1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.
Gene ID: 916 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

Related Products

Description	Clone	Applications

Other Formats

View All CD3 Reagents Request Custom Conjugation

Description	Clone	Applications
APC/Fire™ 750 anti-human CD3	SK7	FC
Biotin anti-human CD3	SK7	FC,ICC
Purified anti-human CD3	SK7	FC,ICC,IHC-F,WB
FITC anti-human CD3	SK7	FC
PE anti-human CD3	SK7	FC
Alexa Fluor® 488 anti-human CD3	SK7	FC
APC anti-human CD3	SK7	FC
PerCP/Cyanine5.5 anti-human CD3	SK7	FC
PerCP anti-human CD3	SK7	FC
PE/Cyanine7 anti-human CD3	SK7	FC
APC/Cyanine7 anti-human CD3	SK7	FC
Alexa Fluor® 700 anti-human CD3	SK7	FC
Pacific Blue™ anti-human CD3	SK7	FC
Alexa Fluor® 647 anti-human CD3	SK7	FC
Brilliant Violet 510™ anti-human CD3	SK7	FC
Brilliant Violet 421™ anti-human CD3	SK7	FC
Brilliant Violet 605™ anti-human CD3	SK7	FC
Brilliant Violet 711™ anti-human CD3	SK7	FC
FITC anti-human CD3	SK7	FC
PE anti-human CD3	SK7	FC
Brilliant Violet 785™ anti-human CD3	SK7	FC
PE/Dazzle™ 594 anti-human CD3	SK7	FC
Brilliant Violet 750™ anti-human CD3	SK7	FC
TotalSeq™-A0049 anti-human CD3	SK7	PG
PerCP/Cyanine5.5 anti-human CD3	SK7	FC
TotalSeq™-C0049 anti-human CD3	SK7	PG
Spark Blue™ 550 anti-human CD3	SK7	FC
PE/Cyanine7 anti-human CD3	SK7	FC
TotalSeq™-B0049 anti-human CD3	SK7	PG
Alexa Fluor® 660 anti-human CD3	SK7	FC
APC/Fire™ 810 anti-human CD3	SK7	FC
Spark NIR™ 685 anti-human CD3	SK7	FC
PE/Fire™ 640 anti-human CD3	SK7	FC
PE/Fire™ 700 anti-human CD3	SK7	FC
APC anti-human CD3	SK7	FC
PerCP anti-human CD3	SK7	FC
APC/Fire™ 750 anti-human CD3	SK7	FC
GMP FITC anti-human CD3	SK7	FC
PE/Cyanine5 anti-human CD3 Antibody	SK7	FC
GMP PE anti-human CD3	SK7	FC
GMP APC anti-human CD3	SK7	FC
GMP PerCP/Cyanine5.5 anti-human CD3	SK7	FC
Spark YG™ 593 anti-human CD3	SK7	FC
GMP PerCP anti-human CD3	SK7	FC
Spark Violet™ 500 anti-human CD3	SK7	FC
Brilliant Violet 650™ anti-human CD3	SK7	FC
Spark Violet™ 500 anti-human CD3	SK7	FC
Spark PLUS UV395™ anti-human CD3	SK7	FC

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.