- Clone
- SK7 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- T3, CD3ε
- Isotype
- Mouse IgG1, κ
- Barcode Sequence
- TATCCCTTGGGATGG
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 344853 | 10 µg | $369.00 | |||
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-B oligomer under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
PG - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-B antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTNNNNNNNNNN (PCR handle), and NNNNNNNNNGCTTTAAGGCCGGTCCTAGC*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) -
- Kan EA, et al. 1983. J. Immunol. 131:536.
- Wood GS, et al. 1985. Am. J. Pathol. 120:371.
- Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
- Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
- Carbone A, et al. 1999. Blood 93:2319. (IHC)
- Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
- Rutjens E, et al. 2007. J. Immunol. 178:1702.
- Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- RRID
-
AB_2860891 (BioLegend Cat. No. 344853)
Antigen Details
- Structure
- Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
- Distribution
-
Mature T and NK T cells, during thymocyte differentiation
- Function
- Antigen recognition, signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen bound to MHC
- Cell Type
- NKT cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, TCRs
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501. - Gene ID
- 916 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
Other Formats
View All CD3 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
APC/Fire™ 750 anti-human CD3
Human peripheral blood lymphocytes were stained with CD19 PE... 
-
Biotin anti-human CD3
Human peripheral blood lymphocytes stained with biotinylated... 
Human PBMCs, stimulated with 1 µg/ml of LPS for 8 h and trea... -
Purified anti-human CD3
Human peripheral blood lymphocytes stained with SK7 FITC. -
FITC anti-human CD3
Human peripheral blood lymphocytes stained with SK7 FITC. -
PE anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
Alexa Fluor® 488 anti-human CD3
Human peripheral blood lymphocytes stained with SK7 Alexa Fl... -
APC anti-human CD3
Human peripheral blood lymphocytes stained with SK7 APC -
PerCP/Cyanine5.5 anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
PerCP anti-human CD3
Human peripheral blood lymphocytes stained with SK7 PerCP -
PE/Cyanine7 anti-human CD3
Human peripheral blood lymphocytes stained with SK7 PE/Cyani... -
APC/Cyanine7 anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
Alexa Fluor® 700 anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
Pacific Blue™ anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
Alexa Fluor® 647 anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
Brilliant Violet 510™ anti-human CD3
Human peripheral blood lymphocytes were stained with SK7 Bri... -
Brilliant Violet 421™ anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
Brilliant Violet 605™ anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
Brilliant Violet 711™ anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
FITC anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
PE anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 785™ anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
PE/Dazzle™ 594 anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
Brilliant Violet 750™ anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
TotalSeq™-A0049 anti-human CD3
-
PerCP/Cyanine5.5 anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-C0049 anti-human CD3
-
Spark Blue™ 550 anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
PE/Cyanine7 anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-B0049 anti-human CD3
-
Alexa Fluor® 660 anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
APC/Fire™ 810 anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
Spark NIR™ 685 anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
PE/Fire™ 640 anti-human CD3
Human peripheral blood lymphocytes were stained with CD3 (cl... -
PE/Fire™ 700 anti-human CD3
Human peripheral blood lymphocytes were stained with anti-hu... -
APC anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
PerCP anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine5 anti-human CD3 Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
GMP PE anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
GMP PerCP/Cyanine5.5 anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
Spark YG™ 593 anti-human CD3
Human peripheral blood lymphocytes were stained with anti-h... -
GMP PerCP anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 500 anti-human CD3
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 650™ anti-human CD3
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Violet™ 500 anti-human CD3
Typical results from human peripheral blood lymphocytes stai... -
Spark PLUS UV395™ anti-human CD3
Human peripheral blood lymphocytes were stained with anti-hu... 
Human peripheral blood lymphocytes were stained with anti-hu...