Phase-Flow™ Alexa Fluor® 647 BrdU Kit

Pricing & Availability
Clone
3D4 (See other available formats)
Regulatory Status
RUO
Other Names
5-bromodeoxyuridine, bromodeoxyuridine
Isotype
Mouse IgG1, κ
Phase-Flow_A647_BrdU_Kit_1_041417
Ramos cells loaded with BrdU for 2 hours (upper panel) or left as no load controls (lower panel) stained using the Phase-Flow™ Alexa Fluor® 647 BrdU Kit and DAPI.
  • Phase-Flow_A647_BrdU_Kit_1_041417
    Ramos cells loaded with BrdU for 2 hours (upper panel) or left as no load controls (lower panel) stained using the Phase-Flow™ Alexa Fluor® 647 BrdU Kit and DAPI.
  • Phase-Flow_A647_BrdU_Kit_2_041417
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Cat # Size Price Quantity Check Availability
370706 100 tests $809.00
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Description

BrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.

Technical data sheet

Kit Contents

Kit Contents
  • BrdU pulsing solution
  • Anti-BrdU antibody
  • Buffer A
  • Buffer B
  • Buffer C
  • DNAse (lyophilized)
  • PBS (Ca2+/Mg2+)
  • 7-AAD
  • DAPI

Product Details

Host Species
Mouse
Immunogen
Iodouridine-conjugated ovalbumin
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Varies by component, see manual for instructions.
Application

ICFC - Quality tested
Recommended Usage

See Manual for instructions.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry and fluorescence microscopy.

Application References

(PubMed link indicates BioLegend citation)
  1. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
  2. Hirota K, et al. 2007. J. Exp. Med. 204:41.
  3. Godebu E, et al. 2008. J. Immunol. 181:1798.
  4. Waskow C, et al. 2008. Nat. Immunol. 9:676.
Product Citations
  1. Hagan N, et al. 2020. Cell Death Dis. 1.086111111. PubMed
  2. Fu Z, et al. 2021. Nat Commun. 12:4462. PubMed
  3. Monlish DA, et al. 2021. JCI Insight. 6:. PubMed
RRID
AB_3097036 (BioLegend Cat. No. 370706)

Antigen Details

Structure
Uridine derivative that can be incorporated into DNA and substitute thymidine residues.
Distribution

Cells can be pulse-labeled with BrdU, which will be incorporated into DNA during the cell cycle's synthesis phase.

Function
The antibody against BrdU can be used to identify cells undergoing DNA replication during the BrdU incorporation period.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Barker JM, et al. 2013. PLoS One 8:e63692.
2. Duque A and Rakic P. 2011. J. Neurosci. 31:15205.
3. Robbins S, et al. 2011. J. Vis. Exp. 55:2855.
4. Broekhuizen CA, et al. 2010. Infect Immun. 78:954.
5. van der Wath RC, et al. 2009. PLoS One 4:e6972.
6. Dolbeare F, et al. 1985. Cytometry 6:521.
7. Gratzner HG. 1982. Science 218:474.

Gene ID
NA
UniProt
View information about BrdU on UniProt.org
Go To Top Version: 1    Revision Date: 04/14/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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