- Clone
- 1A4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Actin, aortic smooth muscle (αSMA), alpha 2 actin (ACTA2), alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein
- Isotype
- Mouse IgG2a, κ
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IHC staining of purified anti-α smooth muscle actin (clone 1A4) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tween-20 (Cat. No. 925501), the tissue was incubated without (panels A and C) and with (panels B and D) 5 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG antibody (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (panels A and B) (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm -
HeLa cells were fixed with ice-cold methanol and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 1.25 µg/mL of purified anti-α smooth muscle actin (clone 1A4), followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 60X objective. Scale bar: 50 µm -
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of purified anti-α smooth muscle actin (clone 1A4) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 614851 | 25 µg | $125.00 | |||
| 614852 | 100 µg | $310.00 | |||
Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins expressed in mammals. α-smooth muscle actin is widely expressed in contractile cells, most notably the mural cells that line arteries and arterioles. Overexpression of α-smooth muscle actin is a hallmark of fibrosis, a thickening or scarring of damaged tissue.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- N-terminal synthetic peptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH).
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
ICC, WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration of 1.25 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Previously Covance catalog# MMS-466S.
When used in ICC testing, we recommend fixing and permeabilizing with ice-cold methanol. - RRID
-
AB_2941620 (BioLegend Cat. No. 614851)
AB_2941620 (BioLegend Cat. No. 614852)
Antigen Details
- Structure
- α smooth muscle actin is a 377 amino acid protein with a predicted molecular weight of 42 kD.
- Distribution
-
Cytoplasmic, cytoskeleton
- Function
- Cell motility
- Interaction
- α smooth muscle actins bind to myosins to produce motive force
- Cell Type
- Mesenchymal Stem Cells, Monocytes
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microfilaments
- Antigen References
-
- Brennan PA, et al. 2000. J Oral Pathol Med. 29:279-83.
- Rizeq MN, et al. 1994. Hum Pathol. 25:671-7.
- Schmitt-Graff A, et al. 1991. Am J Pathol. 138::1233-42
- Roholl PJM, et al. 1990. Hum Pathol. 21:1269-74.
- Alberts B, et al. 1989. Eds Molecular biology of the cell. 2:613.
- Skalli O, et al. 1986. J Cell Biol. 103:2787-96.
- Gene ID
- 59 View all products for this Gene ID
- UniProt
- View information about alpha Smooth Muscle Actin on UniProt.org
Other Formats
View All α Smooth Muscle Actin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Anti-Alpha Smooth Muscle Actin | 1A4 | IHC-P,ICC,WB |
| Purified anti-α Smooth Muscle Actin | 1A4 | IHC-P,ICC,WB |
| Alexa Fluor® 488 anti-α Smooth Muscle Actin | 1A4 | IHC-P,ICC |
| Alexa Fluor® 647 anti-α Smooth Muscle Actin | 1A4 | IHC-P,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-Alpha Smooth Muscle Actin
Immunohistochemical staining of Smooth Muscle Actin (1A4) Mo... -
Purified anti-α Smooth Muscle Actin
IHC staining of purified anti-α smooth muscle actin (clone 1... 
HeLa cells were fixed with ice-cold methanol and blocked wit... 
Whole cell extracts (15 µg total protein) from the indicated... -
Alexa Fluor® 488 anti-α Smooth Muscle Actin
IHC staining of Alexa Fluor® 488 anti-α Smooth Muscle Actin ... 
NIH/3T3 cells were treated with (panels A and B) or without ... -
Alexa Fluor® 647 anti-α Smooth Muscle Actin
IHC staining of Alexa Fluor® 647 anti-α Smooth Muscle Actin ... 
NIH/3T3 cells were treated with (panels A and B) or without ...