Purified anti-human CD20 Maxpar Ready Antibody anti-CD20

Pricing & Availability

Clone: 2H7 (See other available formats)
Regulatory Status: RUO
Workshop: IV B201
Other Names: B1, Bp35
Isotype: Mouse IgG2b, κ

2H7_Purified_CD20_CyTOF_111213 — Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 147m-anti-CD20 (2H7). Data provided by DVS Sciences.

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Size

Price

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302343

100 µg

$129.00

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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca²⁺ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Technical data sheet

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human tonsillar B cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴ and immunohistochemical staining of acetone-fixed frozen sections⁵.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
Polyak MJ, et al. 2002. Blood 99:3256. (IP)
Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)

Product Citations

Fox-Fisher I, et al. 2022. Med (N Y). 3:468. PubMed
Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
NULL, et al. 2022. Cell. 185:916. PubMed
Hwang B, et al. 2021. Nat Methods. 18:903. PubMed

RRID

AB_2562816 (BioLegend Cat. No. 302343)

Antigen Details

Structure: Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution: B cell, T cell subsets
Function: B cell activation
Ligand/Receptor: Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type: B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.
Gene ID: 931 View all products for this Gene ID
UniProt: View information about CD20 on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Other Formats

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Description	Clone	Applications
APC anti-human CD20	2H7	FC
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PE anti-human CD20	2H7	FC
PE/Cyanine5 anti-human CD20	2H7	FC
Purified anti-human CD20	2H7	FC,CyTOF®,IHC-F,IP
APC/Cyanine7 anti-human CD20	2H7	FC
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Alexa Fluor® 488 anti-human CD20	2H7	FC,IHC-F
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Pacific Blue™ anti-human CD20	2H7	FC
Alexa Fluor® 700 anti-human CD20	2H7	FC
PerCP anti-human CD20	2H7	FC
PerCP/Cyanine5.5 anti-human CD20	2H7	FC
Brilliant Violet 421™ anti-human CD20	2H7	FC,IHC-F
Brilliant Violet 570™ anti-human CD20	2H7	FC
Brilliant Violet 605™ anti-human CD20	2H7	FC
Brilliant Violet 650™ anti-human CD20	2H7	FC
Brilliant Violet 785™ anti-human CD20	2H7	FC
Brilliant Violet 510™ anti-human CD20	2H7	FC
Brilliant Violet 711™ anti-human CD20	2H7	FC
Purified anti-human CD20 (Maxpar® Ready)	2H7	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD20	2H7	FC
Biotin anti-human CD20	2H7	FC
APC/Fire™ 750 anti-human CD20	2H7	FC
Alexa Fluor® 594 anti-human CD20	2H7	ICC,IHC-F
FITC anti-human CD20	2H7	FC
Pacific Blue™ anti-human CD20	2H7	FC
APC anti-human CD20	2H7	FC
PE/Cyanine7 anti-human CD20	2H7	FC
TotalSeq™-A0100 anti-human CD20	2H7	PG
TotalSeq™-B0100 anti-human CD20	2H7	PG
TotalSeq™-C0100 anti-human CD20	2H7	PG
PerCP/Cyanine5.5 anti-human CD20	2H7	FC
Spark NIR™ 685 anti-human CD20	2H7	FC
Spark YG™ 593 anti-human CD20	2H7	FC
GMP FITC anti-human CD20	2H7	FC
TotalSeq™-D0100 anti-human CD20	2H7	PG
GMP APC anti-human CD20	2H7	FC
Spark Violet™ 500 anti-human CD20	2H7	FC
GMP Pacific Blue™ anti-human CD20	2H7	FC
GMP PerCP/Cyanine5.5 anti-human CD20	2H7	FC
Spark Violet™ 538 anti-human CD20	2H7	FC
APC/Fire™ 750 anti-human CD20	2H7	FC
PE anti-human CD20	2H7	FC
GMP PE/Cyanine7 anti-human CD20	2H7	FC
Spark Blue™ 515 anti-human CD20	2H7	FC
GMP APC/Fire™ 750 anti-human CD20	2H7	FC
GMP PE anti-human CD20	2H7	FC
Spark Blue™ 550 anti-human CD20 (Flexi-Fluor™)	2H7	FC
Spark Blue™ 574 anti-human CD20 (Flexi-Fluor™)	2H7	FC
Spark Red™ 718 anti-human CD20 (Flexi-Fluor™)	2H7	FC
APC/Fire™ 810 anti-human CD20	2H7	FC
Alexa Fluor® 660 anti-human CD20	2H7	FC

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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