Purified anti-human CD45RB Antibody

Pricing & Availability
Clone
MEM-55 (See other available formats)
Regulatory Status
RUO
Workshop
V CD45.08
Other Names
CD45RB
Isotype
Mouse IgG2b, κ
1_MEM-55_Purified_062707
Human peripheral blood lymphocytes stained with purified MEM-55, followed by anti-mouse IgGs FITC
  • 1_MEM-55_Purified_062707
    Human peripheral blood lymphocytes stained with purified MEM-55, followed by anti-mouse IgGs FITC
  • 2_MEM-55_PURE_CD45RB_2_IHC-P_010318
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate-Buffered 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with 0.5% Tween 20 in PBS and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-human CD45RB antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-mouse IgG (minimal x-reactivity) (clone Poly4053) antibody (red) for an hour. Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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310202 100 µg $112.00
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Description

CD45RB is a 180-240 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes/macrophages, granulocytes, and dendritic cells. CD45RB enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines. The MEM-55 antibody recognizes a silaidase-sensitive epitope of CD45.

Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human thymocytes and T lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The MEM-55 antibody is specific for a glycosylated-CD45RB epitope that is highly expressed on memory B cells and plasmablasts but not on naïve B cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Horejsi V, et al. 1988. Folia Biol. 34:23.
  2. Bazil V, et al. 1989. Immunogenetics 29:202.
  3. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  4. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  5. Koethe S, et al. 2011. J. Leukoc. Biol. 90:5.
Product Citations
  1. Rodriguez L, et al. 2020. Cell Reports Medicine. 1(5):100078. PubMed
  2. Olin A, et al. 2018. Cell. 174:1277. PubMed
  3. Wen J, et al. 2021. PLoS Pathog. 17:e1009738. PubMed
RRID
AB_314805 (BioLegend Cat. No. 310202)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane, 180-240 kD (exon 5 splicing of CD45 gene)
Distribution

Thymocytes, T cell subset, B cells, monocytes/macrophages, granulocytes, dendritic cells

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RB on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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