Purified anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
1-MEL-14_Purified_090707
C57BL/6 mouse splenocytes stained with purified MEL-14, followed by anti-rat IgG FITC
  • 1-MEL-14_Purified_090707
    C57BL/6 mouse splenocytes stained with purified MEL-14, followed by anti-rat IgG FITC
  • 2-MEL-14_pure_CD62L_Antibody_2_103018
    Fresh, frozen mouse spleen was stained with purified CD62L clone MEL-14 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with Ly6c Cy3 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
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Cat # Size Price Quantity Check Availability
104402 500 µg $138.00
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
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  5. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  6. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  7. Ban YH, et al. 2017. Cell Rep. 2.6375. PubMed
  8. Guo R, et al. 2020. Cell Res. 30:21. PubMed
  9. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
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  13. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
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  22. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
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  29. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  30. Kim SJ, et al. 2017. Nat Immunol. 18:1016. PubMed
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  32. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  33. Gil-Pulido J, et al. 2017. PLoS One.. 10.1371/journal.pone.0181947. PubMed
  34. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
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  39. Daley D, et al. 2017. Nat Med. 23:556. PubMed
  40. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  41. Yates K, et al. 2018. Proc Natl Acad Sci U S A. 115:2162. PubMed
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  50. Yi J, et al. 2019. Mol Cells. 42:228. PubMed
  51. Benson M, et al. 2007. J Exp Med. 204:1765. PubMed
  52. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  53. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  54. Jun JC, et al. 2017. PLoS One. 12:e0180688. PubMed
  55. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  56. Olver S, et al. 2005. Cancer Res. 66:571. PubMed
  57. Richards KA, et al. 2018. Front Immunol. 0.829861111. PubMed
  58. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
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  60. Karuppuchamy T, et al. 2020. Inflamm Bowel Dis. 216:26. PubMed
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  62. Managlia E, et al. 2019. Am J Pathol. 189:604. PubMed
  63. Fukushima A, et al. 2006. Invest Ophthalmol Vis Sci. 47:657. PubMed
  64. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
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RRID
AB_313089 (BioLegend Cat. No. 104402)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
UniProt
View information about CD62L on UniProt.org
Go To Top Version: 2    Revision Date: 10/30/2018

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