Purified anti-mouse IL-6 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS-7-expressed, recombinant mouse IL-6

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ELISA Capture - Quality tested
CyTOF® - Verified
ELISPOT Capture, Neut, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA applications, a concentration range of 0.5 – 2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of mouse IL-6 recombinant protein ranging from 500 to 8 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture^1-3,11 or ELISPOT Capture^4,5: The purified MP5-20F3 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MP5-32C11 antibody (Cat. No. 504602) as the detecting antibody and recombinant mouse IL-6 (Cat. No. 575709) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Flow Cytometry: The fluorochrome-labeled MP5-20F3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-6 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.

Neutralization^1-2,8-10: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-6 bioactivity in vivo and in vitro (Cat. No. 504506).

Additional reported applications (for relevant formats of this clone) include: In Vivo Capture⁷, and immunohistochemical staining (paraformaldehyde-fixed/saponin-treated⁶, and acetone-fixed frozen tissue sections^{13, 14}).

Note: For testing mouse IL-6 in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 431301 to 431306) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)

1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA Capture, Neut)
3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA Capture)
4. Fujihashi K, et al. 1991. J. Immunol. Meth. 160:181. (ELISPOT Capture)
5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT Capture)
6. Litton M, et al. 1994. J. Immunol Meth. 175:47. (IHC)
7. Finkelman F, et al. 2003. Curr. Prot. Immunol. John Wiley & Sons, New York. Unit 6.28. (In Vivo Capture)
8. Starnes HF Jr, et al. 1990. J. Immunol. 145:4185. (Neut)
9. Riedemann NC, et al. 2003. J. Immunol. 170:503. (Neut)
10. Rochman I, et al. 2005. J. Immunol. 174:4761. (Neut)
11. O'Connell PJ, et al. 2006. Blood 107:1010. (ELISA Capture)
12. Ozeki Y, et al. 2010. Int. Immunol. 22:179. PubMed
13. Galie M, et al. 2005. Carcinogenesis 26:1868 (IHC)
14. Schone MP, et al. 2000. J Immunol 165:6583 (IHC)
15. Keswani T, et al. 2014. Exp Parasitol. 141:82. PubMed
16. Notley CA, et al. 2015. J Immunol. 194:1621. PubMed

Product Citations

1. Bahreyni A, et al. 2023. Biomed Pharmacother. 163:114789. PubMed
2. Bahreyni A, et al. 2023. BMC Med. 21:193. PubMed
3. Wang J, et al. 2023. Elife. 12:. PubMed
4. Wu G, et al. 2020. Front Oncol. 10:194. PubMed
5. Crawford G, et al. 2018. Nat Immunol. 1.388194444. PubMed
6. Xu M et al. 2018. Immunity. 48(4):787-798 . PubMed
7. Lee J, et al. 2015. PLoS One. 10: 0142624. PubMed
8. Al Sayed MF, et al. 2019. Cancer Res. 79:346. PubMed
9. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
10. Morita M, et al. 2021. Heliyon. 7:e06228. PubMed
11. Muraleedharan A, et al. 2021. Glia. 69:697. PubMed
12. Schwaderer J, et al. 2020. Cell Death Dis. 11:154. PubMed
13. Peterson LW, et al. 2017. J Exp Med. 214:3171. PubMed
14. Herrada AA, et al. 2022. Front Immunol. 13:841641. PubMed
15. Daley D, et al. 2017. Nat Med. 23:556. PubMed
16. Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed
17. Di Conza G, et al. 2021. Nat Immunol. 22:1403. PubMed
18. Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed
19. Song K, et al. 2017. Oncotarget. 8:38554. PubMed
20. Ji Z, et al. 2019. Front Immunol. 1.722916667. PubMed
21. Ozeki Y, et al. 2010. Int Immunol. 1.040972222. PubMed
22. Rosen DA, et al. 2019. Sci Transl Med. 11:eaau5266. PubMed
23. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed

RRID

AB_315335 (BioLegend Cat. No. 504501)
AB_315335 (BioLegend Cat. No. 504502)

Antigen Details

Structure

Neuropoietic cytokine family; 22-29 kD (Mammalian)

Bioactivity

Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction

Cell Sources

T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells

Cell Targets

T cells, B cells, hepatocytes, cholinergic neurons

Receptors

Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)

Biology Area

Immunology, Innate Immunity

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers M, et al. 1993. Ann. Hematology 66:219.

Regulation

Upregulated by IL-1, TNF, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β

Gene ID

16193 View all products for this Gene ID

UniProt

View information about IL-6 on UniProt.org

Related Protocols

• Active Protocols: Sandwich ELISA - Video
• Sandwich ELISA Protocol

Other Formats