- Clone
- 2B8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- c-KIT, Stem Cell Factor Receptor (SCFR)
- Isotype
- Rat IgG2b, κ
| Cat # | Size | Price | Quantity | |||
|---|---|---|---|---|---|---|
| 285154 | 50 µg | $336.00 | ||||
CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.
Product Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse bone marrow mast cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 574 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Blue Laser (488 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
-
Application References
(PubMed link indicates BioLegend citation) -
- Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
- Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
- Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- RRID
-
AB_3662494 (BioLegend Cat. No. 285154)
Antigen Details
- Structure
- Ig superfamily, 145 kD
- Distribution
-
Hematopoietic stem cells, AML, mast cells
- Function
- Growth factor receptor, tyrosine kinase
- Ligand/Receptor
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5. - Gene ID
- NA
- UniProt
- View information about CD117 on UniProt.org
Other Formats
View All CD117 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse CD117 (c-Kit)
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Biotin anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with B220 APC a... 
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FITC anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells were stained with CD45R/B220 PE an... 
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PE anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC -
PE/Cyanine5 anti-mouse CD117 (c-Kit)
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Purified anti-mouse CD117 (c-Kit)
C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC -
PE/Cyanine7 anti-mouse CD117 (c-Kit)
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b... -
Alexa Fluor® 488 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... 
C57BL/6 mouse frozen testis section was fixed with 4% parafo... -
Alexa Fluor® 647 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... 
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... 
C57BL/6 mouse frozen testis section was fixed with 4% parafo... -
Pacific Blue™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... 
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... -
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells stained with CD45R/B220 (clo... -
PerCP anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... 
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2)... -
APC/Cyanine7 anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Brilliant Violet 421™ anti-mouse CD117 (c-Kit)
Confocal image of C57BL/6 mouse small intestine sample acqui... 
Confocal image of human lymph node sample acquired using the... -
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready)
Mouse bone marrow cells stained with 164Dy anti-Sca-1 (D7) a... -
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... 
-
Brilliant Violet 711™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... 
-
Alexa Fluor® 594 anti-mouse CD117 (c-Kit)
C57BL/6 mouse frozen testis section was fixed with 4% parafo... 
Paraformaldehyde-fixed (4%), 500 µm-thick mouse testis secti... -
APC/Fire™ 750 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... 
-
Brilliant Violet 510™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b Alex... 
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Brilliant Violet 785™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b Alex... 
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TotalSeq™-A0012 anti-mouse CD117 (c-kit)
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Brilliant Violet 605™ anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Alexa Fluor® 700 anti-mouse CD117 (c-Kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
TotalSeq™-B0012 anti-mouse CD117 (c-Kit)
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TotalSeq™-C0012 anti-mouse CD117 (c-Kit)
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Spark NIR™ 685 anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Brilliant Violet 650™ anti-mouse CD117 (c-kit)
C57BL/6 mouse bone marrow cells were stained with CD11b FITC... -
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™)
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Spark Blue™ 574 anti-mouse CD117 (c-kit) (Flexi-Fluor™)
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Spark Blue™ 550 anti-mouse CD117 (c-kit) (Flexi-Fluor™)