Spark Red™ 718 anti-human IgM (Flexi-Fluor™) Antibody, IgM, MHM-88

Pricing & Availability

Clone: MHM-88 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG1, κ

Compare all formats See Spark Red™ 718 spectral data

Cat #

Size

Price

Quantity

285092

50 µg

$375.00

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Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Technical data sheet

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human Ig cocktail

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.

Concentration

0.2 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

Recommended Usage

Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

Excitation Laser

Red Laser (633 nm)

Application Notes

MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Application References (PubMed link indicates BioLegend citation)

Perez-Shiyama C, et al. 2014. J Immunol. 192:5192. PubMed

RRID

AB_3106347 (BioLegend Cat. No. 285092)

Antigen Details

Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3507 View all products for this Gene ID
UniProt: View information about IgM on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Other Formats

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Description	Clone	Applications
Purified anti-human IgM	MHM-88	FC,ELISA
PE anti-human IgM	MHM-88	FC
Biotin anti-human IgM	MHM-88	FC,ELISA
FITC anti-human IgM	MHM-88	FC
APC anti-human IgM	MHM-88	FC
PerCP/Cyanine5.5 anti-human IgM	MHM-88	FC
Pacific Blue™ anti-human IgM	MHM-88	FC
Brilliant Violet 421™ anti-human IgM	MHM-88	FC
APC/Cyanine7 anti-human IgM	MHM-88	FC
Brilliant Violet 570™ anti-human IgM	MHM-88	FC
Brilliant Violet 510™ anti-human IgM	MHM-88	FC
Brilliant Violet 605™ anti-human IgM	MHM-88	FC
Brilliant Violet 650™ anti-human IgM	MHM-88	FC
Purified anti-human IgM (Maxpar® Ready)	MHM-88	FC,CyTOF®
PE/Dazzle™ 594 anti-human IgM	MHM-88	FC
PE/Cyanine7 anti-human IgM	MHM-88	FC
Alexa Fluor® 488 anti-human IgM	MHM-88	FC
Alexa Fluor® 647 anti-human IgM	MHM-88	FC
Alexa Fluor® 700 anti-human IgM	MHM-88	FC
Brilliant Violet 711™ anti-human IgM	MHM-88	FC
TotalSeq™-A0136 anti-human IgM	MHM-88	PG
TotalSeq™-C0136 anti-human IgM	MHM-88	PG
Brilliant Violet 785™ anti-human IgM	MHM-88	FC
APC/Fire™ 750 anti-human IgM	MHM-88	FC
TotalSeq™-B0136 anti-human IgM	MHM-88	PG
Spark Violet™ 423 anti-human IgM Antibody	MHM-88	FC
TotalSeq™-D0136 anti-human IgM	MHM-88	PG
Spark Blue™ 550 anti-human IgM	MHM-88	FC
Spark Blue™ 515 anti-human IgM	MHM-88	FC
PE/Fire™ 700 anti-human IgM	MHM-88	FC
Spark YG™ 593 anti-human IgM	MHM-88	FC
PE/Fire™ 640 anti-human IgM	MHM-88	FC
Spark Red™ 718 anti-human IgM (Flexi-Fluor™)	MHM-88	FC
APC anti-human IgM	MHM-88	FC
PE anti-human IgM	MHM-88	FC
Brilliant Violet 750™ anti-human IgM	MHM-88	FC

Go To Top Version: 1 Revision Date: 02/07/2024

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.