- Clone
- HIT2 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- III 155
- Other Names
- T10, ADP-ribosyl cyclase
- Isotype
- Mouse IgG1, κ
- Barcode Sequence
- TGTACCCGCTTGTGA
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 303543 | 10 µg | $369.00 | |||
CD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscles, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Horse, Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
- Preparation
- The antibody was purified by chromatography and conjugated with TotalSeq™-C oligomer under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-C antibodies are compatible with 10x Genomics Chromium Single Cell Immune Profiling Solution.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform. - Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and spatial biology (IBEX)10,11.
- Additional Product Notes
-
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The barcode flanking sequences are CGGAGATGTGTATAAGAGACAGNNNNNNNNNN (PCR handle), and NNNNNNNNNCCCATATAAGA*A*A (capture sequence). N represents either randomly selected A, C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
View more applications data for this product in our Scientific Poster Library. -
Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Dieu M. 1998. J. Exp. Med. 188:373.
- Esser M, et al. 2001. J. Virol. 75:6173.
- Jeannin P, et al. 1999. J. Immunol. 162:2044.
- Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107.
- van der Voort R, et al. 1997. J. Exp. Med. 185:2121. (IHC)
- Bende RJ, et al. 2003. Am. J. Pathol. 162:105.
- Lehner M, et al. 2008. J. Leukoc. Biol. 83:883. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_2800758 (BioLegend Cat. No. 303543)
Antigen Details
- Structure
- ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD
- Distribution
-
T cells, B cells, NK, myeloid, plasma, and dendritic cells
- Function
- Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation
- Ligand/Receptor
- CD31, hyaluronic acid
- Cell Type
- B cells, Dendritic cells, NK cells, Plasma cells, T cells
- Biology Area
- Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Ferrero E, et al. 1999. J. Leukoc. Biol. 65:151.
2. Lund F, et al. 1995. Immunol. Today 16:469. - Gene ID
- 952 View all products for this Gene ID
- UniProt
- View information about CD38 on UniProt.org
Other Formats
View All CD38 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 APC -
FITC anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 FITC -
PE anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PE -
PE/Cyanine5 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PE/Cyan... -
Purified anti-human CD38
Human peripheral blood lymphocytes stained with purified HIT... -
Alexa Fluor® 488 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 Alexa F... -
Alexa Fluor® 647 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 Alexa F... -
PE/Cyanine7 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PE/Cyan... -
Biotin anti-human CD38
Human peripheral blood lymphocytes stained with biotinylated... -
PerCP anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 PerCP -
PerCP/Cyanine5.5 anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Alexa Fluor® 700 anti-human CD38
Human peripheral blood lymphocytes stained with HIT2 Alexa F... 
Confocal image of human lymph node sample acquired using the... -
Brilliant Violet 421™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Brilliant Violet 711™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Brilliant Violet 785™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Brilliant Violet 605™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
APC/Cyanine7 anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Purified anti-human CD38 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 167Er-an... -
PE/Dazzle™ 594 anti-human CD38
Human peripheral blood granulocytes were stained with CD38 (... -
PE anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 510™ anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
FITC anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
PE/Dazzle™ 594 anti-human CD38
Typical results from human peripheral blood granulocytes sta... -
TotalSeq™-A0389 anti-human CD38
-
TotalSeq™-C0389 anti-human CD38
-
APC/Fire™ 750 anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (C... -
TotalSeq™-B0389 anti-human CD38
-
APC/Fire™ 810 anti-human CD38
Human peripheral blood lymphocytes were stained with CD38 (c... -
Spark NIR™ 685 anti-human CD38 Antibody
Human peripheral blood lymphocytes were stained with CD38 (c... -
TotalSeq™-D0389 anti-human CD38
-
PerCP/Cyanine5.5 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
APC anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
PE/Cyanine7 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP PE anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP FITC anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
Pacific Blue™ anti-human CD38
Human peripheral blood lymphocytes were stained with anti-hu... -
Pacific Blue™ anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP APC/Fire™ 750 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Dazzle™ 594 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP APC anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
GMP PE/Cyanine7 anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
Spark Blue™ 515 anti-human CD38
Human peripheral blood cells were surface stained with (left... -
GMP Pacific Blue™ anti-human CD38
Typical results from human peripheral blood lymphocytes stai... -
Spark Violet™ 423 anti-human CD38
Human peripheral blood lymphocytes were stained with anti-hu... -
Brilliant Violet 650™ anti-human CD38
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark PLUS B550™ anti-human CD38
Human peripheral blood lymphocytes were stained with anti-hu...