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Brilliant Violet 605™ anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
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Product Citations
publications
HCD56_BV605_013112
Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 605™.
  • HCD56_BV605_013112
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD56 (clone HCD56) Brilliant Violet 605™.
Compare all formats See Brilliant Violet 605™ spectral data
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318333 25 tests $248
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318334 100 tests $429
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  2. Kim MY, et al. 2021. JCI Insight. 6:. PubMed
  3. Zhang T, et al. 2022. Am J Reprod Immunol. 87:e13524. PubMed
  4. Macedo AB, et al. 2022. J Virol. 96:e0037222. PubMed
  5. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  6. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  7. Mashayekhi M, et al. 2022. Prostaglandins Other Lipid Mediat. 158:106604. PubMed
  8. Metelo AM, et al. 2022. Cancer Res Commun. 2:158. PubMed
  9. Kritikou J, et al. 2016. Sci Rep. 6:30636. PubMed
  10. Bigley A, et al. 2013. Brain Behav Immun. 889:1591. PubMed
  11. Klichinsky M, et al. 2020. Nat Biotechnol. 38:947. PubMed
  12. Sorensen ES, et al. 2020. Antimicrob Agents Chemother. 64:00:00. PubMed
  13. Martrus G, et al. 2017. PLoS One.. 10.1371/journal.pone.0182532. PubMed
  14. Grenga I, et al. 2016. Clin Transl Immunology. 0.265972222. PubMed
  15. Kip P, et al. 2021. Nutrients. 13:. PubMed
  16. Grove K, et al. 2016. PLoS One. 11: 0145961. PubMed
  17. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  18. Jackson E, et al. 2017. PLoS One. 10.1371/journal.pone.0185160. PubMed
  19. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
  20. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  21. Du J, et al. 2014. Cancer Immunol Res. 2:878. PubMed
  22. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  23. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  24. Hardman CS, et al. 2021. Sci Immunol. 6:. PubMed
  25. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
  26. Kinloch NN, et al. 2021. Nat Commun. 12:165. PubMed
  27. Jansen D, et al. 2015. Rheumatology. 54:728. PubMed
  28. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  29. Hernndez DC, et al. 2021. Immunity. :. PubMed
  30. Rydbirk R, et al. 2019. Sci Rep. 9:7781. PubMed
  31. Garfall AL, et al. 2018. JCI Insight. 3. PubMed
  32. Kroloff M, et al. 2022. J Immunother Cancer. 10:. PubMed
  33. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
RRID
AB_2561912 (BioLegend Cat. No. 318333)
AB_2561912 (BioLegend Cat. No. 318334)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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