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FITC anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136
C57BL/6 mouse splenocytes were stained with NK1.1 (clone PK136) FITC and NKG2D PE.
  • PK136
    C57BL/6 mouse splenocytes were stained with NK1.1 (clone PK136) FITC and NKG2D PE.
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108705 50 µg $47
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108706 500 µg $132
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed
  2. Corral D, et al. 2022. Cell Rep. 39:110715. PubMed
  3. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  4. Nozaki K, et al. 2022. Nature. 606:960. PubMed
  5. Aghayev T, et al. 2022. Cancer Discov. 12:1960. PubMed
  6. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  7. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  8. Tsioti I, et al. 2023. J Neuroinflammation. 20:25. PubMed
  9. Wight AE, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200757119. PubMed
  10. Zaman R, et al. 2021. Immunity. :. PubMed
  11. Pandey SP, et al. 2022. Cell Host Microbe. 30:1003. PubMed
  12. Zimmerman SM, et al. 2022. Oncogene. 41:4983. PubMed
  13. Sanmarco LM, et al. 2022. Nature. 611:801. PubMed
  14. Peng J, et al. 2023. Am J Reprod Immunol. 89:e13678. PubMed
  15. Gilleron J et al. 2018. Cell reports. 25(12):3329-3341 . PubMed
  16. Leier A, et al. 2022. Mol Ther Nucleic Acids. 28:261. PubMed
  17. Chung YM, et al. 2022. Am J Cancer Res. 12:1241. PubMed
  18. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  19. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  20. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  21. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  22. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  23. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  24. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  25. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  26. Fullertona A, et al. 2013. Toxicol Appl Pharmacol. 266:317. PubMed
  27. Li X, et al. 2014. PLoS One. 9:108192. PubMed
  28. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
  29. Sodji QH, et al. 2022. Cancer Res Commun. 2:725. PubMed
  30. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  31. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  32. Köchl R, et al. 2020. Elife. 9:00. PubMed
  33. Song Y, et al. 2016. Mol Cancer Ther. 15: 2413 - 2421. PubMed
  34. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  35. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  36. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  37. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  38. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  39. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  40. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  41. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  42. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  43. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  44. Chung YM, et al. 2021. J Immunother Cancer. 9:. PubMed
  45. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  46. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  47. QT N, et al. 2015. Antimicrob Agents Chemother. 59: 6308-6316. PubMed
  48. Wolf KG, et al. 2022. JVS Vasc Sci. 3:336. PubMed
  49. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  50. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  51. Giampazolias E, et al. 2021. Cell. . PubMed
  52. Robinett RA et al. 2018. Cell systems. 7(1):41-48 . PubMed
  53. Shahzad KA, et al. 2018. Drug Deliv. 25:703. PubMed
  54. Zhang W, et al. 2019. Mar Drugs. 0.845138889. PubMed
  55. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  56. Bronisz–Budzynska I, et al. 2019. Skelet Muscle. 9:22. PubMed
  57. Lam KC, et al. 2021. Cell. 184:5338. PubMed
  58. Ruscher R, et al. 2018. Bio Protoc. 8:e2757. PubMed
  59. Nam J, et al. 2021. Adv Ther (Weinh). 4:. PubMed
  60. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  61. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
  62. Yuan C, et al. 2015. Biochem Biophys Res Commun. 464: 249-255. PubMed
  63. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  64. Werner A, et al. 2021. iScience. 24:103076. PubMed
  65. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  66. Jia H, et al. 2018. Int J Oncol. 53:949. PubMed
  67. Bárcena C et al. 2019. EBioMedicine. 43:513-524 . PubMed
  68. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  69. Wang X, et al. 2022. Elife. 11:. PubMed
  70. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  71. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  72. Chen J, et al. 2014. Cell Res. 24:1050. PubMed
  73. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  74. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  75. Sun D, et al. 2020. PLoS Pathog. 16:e1008361. PubMed
  76. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
RRID
AB_313392 (BioLegend Cat. No. 108705)
AB_313392 (BioLegend Cat. No. 108706)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Other Formats

View All NK-1.1 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse NK-1.1 PK136 FC
Biotin anti-mouse NK-1.1 PK136 FC
FITC anti-mouse NK-1.1 PK136 FC
PE anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
PE/Cyanine7 anti-mouse NK-1.1 PK136 FC
PE/Cyanine5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 488 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 647 anti-mouse NK-1.1 PK136 FC
Pacific Blue™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 711™ anti-mouse NK-1.1 PK136 FC
APC/Cyanine7 anti-mouse NK-1.1 PK136 FC
PerCP anti-mouse NK-1.1 PK136 FC
PerCP/Cyanine5.5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 700 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 421™ anti-mouse NK-1.1 PK136 FC,SB
Brilliant Violet 570™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 650™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 510™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 605™ anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 (Maxpar® Ready) PK136 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 785™ anti-mouse NK-1.1 PK136 FC
APC/Fire™ 750 anti-mouse NK-1.1 PK136 FC
TotalSeq™-A0118 anti-mouse NK-1.1 PK136 PG
Ultra-LEAF™ Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
TotalSeq™-B0118 anti-mouse NK-1.1 PK136 PG
TotalSeq™-C0118 anti-mouse NK-1.1 PK136 PG
PE/Fire™ 810 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 640 anti-mouse NK-1.1 PK136 FC
Spark UV™ 387 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 700 anti-mouse NK-1.1 PK136 FC
Go To Top Version: 2    Revision Date: 12/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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