Loading...

PE anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
PK136_PE_NK-11_Antibody_051018
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and NK1.1 (clone PK136) PE (left) or mouse IgG2a, κ PE isotype control (right).
  • PK136_PE_NK-11_Antibody_051018
    C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and NK1.1 (clone PK136) PE (left) or mouse IgG2a, κ PE isotype control (right).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
108707 50 µg $61
Check Availability


Need larger quantities of this item?
Request Bulk Quote
108708 200 µg $179
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Xing J, et al. 2021. Cell Reports. 35(12):109205. PubMed
  2. Calabrese DR, et al. 2020. J Clin Invest. . PubMed
  3. Sade-Feldman M,et al. 2017. Nat Commun.. 10.1038/s41467-017-01062-w. PubMed
  4. Glasner A,et al. 2017. Sci Rep.. 10.1038/s41598-017-12998-w. PubMed
  5. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  6. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  7. Hiyoshi H, et al. 2022. Cell Host Microbe. 30:163. PubMed
  8. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  9. West HC, et al. 2022. Cell Rep. 39:110819. PubMed
  10. Aghayev T, et al. 2022. Cancer Discov. 12:1960. PubMed
  11. Jiang X, et al. 2023. J Clin Invest. 133:. PubMed
  12. Gupta T, et al. 2023. Front Immunol. 14:1044703. PubMed
  13. Kaltenbach L, et al. 2023. Nat Immunol. 24:915. PubMed
  14. Lee Y, et al. 2020. J Innate Immun. 1:. PubMed
  15. Israelow B, et al. 2020. bioRxiv. . PubMed
  16. Gonalves S, et al. 2021. Cell Reports. 34(11):108860. PubMed
  17. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  18. Zheng M, et al. 2016. Sci Rep. 6:28729. PubMed
  19. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  20. Valencia–Hernandez AM, et al. 2020. Cell Host & Microbe. 27(6):950-962. PubMed
  21. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  22. Zhang R, et al. 2019. Cell Rep. 28:2647. PubMed
  23. Iwamoto H et al. 2018. Cell metabolism. 28(1):104-117 . PubMed
  24. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  25. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  26. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  27. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  28. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  29. Adomati T, et al. 2020. Cell Reports. 30(11):3671-3681. PubMed
  30. Hsieh M, et al. 2006. J Immunol. 177:1855. PubMed
  31. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  32. Liu X, et al. 2020. Nature. . PubMed
  33. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  34. Lee AK, et al. 2020. Radiat Res. 193:305. PubMed
  35. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  36. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  37. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  38. Fitzgerald AA, et al. 2021. J Immunother Cancer. 9:. PubMed
  39. Shibata K, et al. 2014. J Immunol. 192:2210. PubMed
  40. Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
  41. Shemer A, et al. 2020. Immunity. 53(5):1033-1049.e7. PubMed
  42. Nakagawa S et al. 2017. Cell host & microbe. 22(5):667-677 . PubMed
  43. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  44. Astrakhan A, et al. 2009. J Immunol. 182:7370. PubMed
  45. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  46. Almutairi F, et al. 2021. Front Immunol. 12:772288. PubMed
  47. Wang LT, et al. 2021. STAR Protocols. 2(1):100337. PubMed
  48. Marsh S, et al. 2016. Proc Natl Acad Sci U S A. 113: 1316 - 1325. PubMed
  49. Spees A, et al. 2014. Infect Immun . 82:1692. PubMed
  50. Chen Y, et al. 2012. PLoS One. 7:e47190. PubMed
  51. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  52. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  53. Lee M, et al. 2014. PLoS One. 9:112666. PubMed
  54. Li J, et al. 2019. JCI Insight. 5. PubMed
  55. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  56. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  57. Martens CR, et al. 2022. PLoS Pathog. 18:e1010342. PubMed
  58. Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed
  59. Qiu Z, et al. 2016. Sci Rep. 6:31936. PubMed
  60. Poczobutt J, et al. 2016. J Immunol. 196: 891 - 901. PubMed
  61. Hiramoto T, et al. 2018. Mol Ther. 26:1255. PubMed
  62. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  63. Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed
  64. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  65. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  66. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  67. Galvani E, et al. 2020. Nat Commun. 11:853. PubMed
  68. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  69. Ulrich-Lewis JT, et al. 2022. Front Immunol. 13:861710. PubMed
  70. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  71. Huang B, et al. 2015. PLoS One. 10: 0134715. PubMed
  72. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  73. Markman JL, et al. 2020. Nat Commun. 11:1613. PubMed
  74. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  75. Tang X, et al. 2022. Cell Rep. 41:111673. PubMed
  76. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  77. Kadowaki T, et al. 2012. Clin Immunol. 142:296. PubMed
  78. Olson M, et al. 2014. J Immunol. 193:5420. PubMed
  79. Kim DH, et al. 2018. Nat Commun. 9:503. PubMed
  80. Li Z, et al. 2018. Front Immunol. 9:347. PubMed
  81. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  82. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  83. Duan B, et al. 2010. J Immunol. 185:335. PubMed
  84. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  85. Shannon JP, et al. 2021. STAR Protoc. 2:100790. PubMed
  86. Sun C, et al. 2022. J Adv Res. 35:71. PubMed
  87. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  88. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  89. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  90. Korrer M, Routes J 2014. PLoS One. 9:91370. PubMed
  91. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  92. Bachu M, et al. 2019. J Biol Chem. 294:1904. PubMed
  93. Wu S, et al. 2022. BMC Immunol. 23:17. PubMed
  94. Lang V, et al. 2021. Elife. 10:. PubMed
  95. Bates PD, et al. 2021. Front Immunol. 12:668307. PubMed
  96. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  97. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  98. Muthalagu N, et al. 2020. Cancer Discov. 1.022222222. PubMed
  99. Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed
  100. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  101. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  102. Zhang X, et al. 2021. Mol Cancer Res. 19:1076. PubMed
  103. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  104. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
  105. Wang LT, et al. 2020. Cell Rep. 32:108188. PubMed
  106. Lokken K, et al. 2014. PLoS Pathog. 10:1004049. PubMed
  107. Hohsfield LA, et al. 2020. J Neuroinflammation. 0.902083333. PubMed
  108. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  109. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  110. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  111. Okamoto T, et al. 2011. Biomaterials. 32:4925. PubMed
  112. Kitur K, et al. 2015. PLoS Pathog. 11:1004820. PubMed
  113. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  114. Ovadya Y, et al. 2018. Nat Commun. 9:5435. PubMed
  115. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  116. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  117. He B, et al. 2020. Cell Reports. 30(3):714-724.e5.. PubMed
  118. Resch TK, et al. 2018. Sci Rep. 0.722916667. PubMed
  119. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  120. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  121. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  122. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  123. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  124. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  125. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  126. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  127. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  128. Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed
  129. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  130. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  131. Orr MT, et al. 2019. NPJ Vaccines. 4:1. PubMed
  132. Sartorius R, et al. 2018. Front Immunol. 1.413888889. PubMed
  133. Zhou X, et al. 2021. Nutr Metab (Lond). 18:64. PubMed
  134. Han J, et al. 2015. Cancer Res . 75: 5273 - 5282. PubMed
  135. Dienz O, et al. 2009. J Exp Med. 206:69. PubMed
  136. Wu C, et al. 2018. Scand J Immunol. 87:e12661. PubMed
  137. Hohsfield LA, et al. 2021. Elife. 10:. PubMed
  138. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  139. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
RRID
AB_313394 (BioLegend Cat. No. 108707)
AB_313394 (BioLegend Cat. No. 108708)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All NK-1.1 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse NK-1.1 PK136 FC
Biotin anti-mouse NK-1.1 PK136 FC
FITC anti-mouse NK-1.1 PK136 FC
PE anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
PE/Cyanine7 anti-mouse NK-1.1 PK136 FC
PE/Cyanine5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 488 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 647 anti-mouse NK-1.1 PK136 FC
Pacific Blue™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 711™ anti-mouse NK-1.1 PK136 FC
APC/Cyanine7 anti-mouse NK-1.1 PK136 FC
PerCP anti-mouse NK-1.1 PK136 FC
PerCP/Cyanine5.5 anti-mouse NK-1.1 PK136 FC
Alexa Fluor® 700 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 421™ anti-mouse NK-1.1 PK136 FC,SB
Brilliant Violet 570™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 650™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 510™ anti-mouse NK-1.1 PK136 FC
Brilliant Violet 605™ anti-mouse NK-1.1 PK136 FC
Purified anti-mouse NK-1.1 (Maxpar® Ready) PK136 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse NK-1.1 PK136 FC
Brilliant Violet 785™ anti-mouse NK-1.1 PK136 FC
APC/Fire™ 750 anti-mouse NK-1.1 PK136 FC
TotalSeq™-A0118 anti-mouse NK-1.1 PK136 PG
Ultra-LEAF™ Purified anti-mouse NK-1.1 PK136 FC,CyTOF®,IP,Depletion,Block,IHC-F
TotalSeq™-B0118 anti-mouse NK-1.1 PK136 PG
TotalSeq™-C0118 anti-mouse NK-1.1 PK136 PG
PE/Fire™ 810 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 640 anti-mouse NK-1.1 PK136 FC
Spark UV™ 387 anti-mouse NK-1.1 PK136 FC
PE/Fire™ 700 anti-mouse NK-1.1 PK136 FC
Go To Top Version: 3    Revision Date: 05/10/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account