Loading...

PE anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104
BALB/c (solid line) and SJL (broken line) splenocytes stained with 104 PE
  • 104
    BALB/c (solid line) and SJL (broken line) splenocytes stained with 104 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
109807 50 µg $61
Check Availability


Need larger quantities of this item?
Request Bulk Quote
109808 200 µg $214
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  2. Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed
  3. Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed
  4. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  5. He D, et al. 2022. Immunity. 55:159. PubMed
  6. Wang H, et al. 2022. Immunol Cell Biol. 100:547. PubMed
  7. Nozaki K, et al. 2022. Nature. 606:960. PubMed
  8. Shemesh A, et al. 2022. J Exp Med. 219: . PubMed
  9. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  10. Nishina T, et al. 2023. iScience. 26:105934. PubMed
  11. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  12. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  13. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  14. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  15. Melzi E, et al. 2022. Immunity. 55:2168. PubMed
  16. Yi C, et al. 2023. J Neuroinflammation. 20:45. PubMed
  17. Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed
  18. Oh J, et al. 2023. Nat Commun. 14:3278. PubMed
  19. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  20. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  21. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  22. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  23. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  24. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  25. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  26. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  27. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  28. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  29. Davila S, et al. 2014. J Immunol. 192:4284. PubMed
  30. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  31. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  32. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  33. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  34. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  35. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  36. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  37. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  38. Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
  39. Amor C, et al. 2020. Nature. 583:127. PubMed
  40. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  41. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  42. Rengarajan S, et al. 2020. Cell Rep Med. :1. PubMed
  43. He L, et al. 2019. iScience. 0.790972222. PubMed
  44. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  45. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  46. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  47. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  48. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  49. Chen D, et al. 2021. Molecular Cell. 81:3833. PubMed
  50. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  51. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  52. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  53. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  54. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  55. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  56. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  57. Ndeupen S, et al. 2022. STAR Protoc. 3:101350. PubMed
  58. Zhang X, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  59. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  60. Tang Q, et al. 2021. Immunity. 54:1961. PubMed
  61. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  62. Hirata T, et al. 2012. Int Immunol. 24:705. PubMed
  63. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  64. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  65. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  66. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  67. Xiong J, et al. 2011. Int Immunol. 23:56. PubMed
  68. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  69. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  70. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  71. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  72. Wang X, et al. 2021. Cell. 184:5357. PubMed
  73. Koide S, et al. 2022. iScience. 25:103603. PubMed
  74. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  75. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  76. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  77. Sindrilaru A, et al. 2009. Blood. 113:5266. PubMed
  78. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  79. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  80. Wang X, et al. 2022. Elife. 11:. PubMed
  81. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  82. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  83. Huang X, et al. 2021. Immunity. . PubMed
  84. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  85. Spath S, et al. 2022. iScience. 25:104998. PubMed
  86. Young K, et al. 2021. Cell Stem Cell. . PubMed
  87. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  88. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  89. Finkin S et al. 2019. Immunity. 51(2):324-336 . PubMed
  90. Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed
  91. Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed
  92. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  93. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
  94. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
RRID
AB_313444 (BioLegend Cat. No. 109807)
AB_313444 (BioLegend Cat. No. 109808)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account