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PE/Cyanine7 anti-mouse/human CD44 Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
IM7_PECy7_011108
C57BL/6 mouse splenocytes stained with IM7 PE/Cyanine7
  • IM7_PECy7_011108
    C57BL/6 mouse splenocytes stained with IM7 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103029 25 µg $94
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103030 100 µg $215
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang Y, et al. 2023. J Nanobiotechnology. 21:109. PubMed
  2. del Rio ML, et al. 2023. Front Immunol. 14:1113858. PubMed
  3. Wang X, et al. 2023. Nat Commun. 14:3440. PubMed
  4. Liu Y, et al. 2023. Nat Commun. 14:3745. PubMed
  5. Furrer R, et al. 2021. Sci Adv. 7:eabi4852. PubMed
  6. Kragten NAM, et al. 2022. Eur J Immunol. 52:389. PubMed
  7. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  8. Koenig A, et al. 2022. Front Immunol. 12:791100. PubMed
  9. Parga-Vidal L, et al. 2022. Eur J Immunol. 52:1095. PubMed
  10. Liedmann S, et al. 2022. Mol Cell. 82:2401. PubMed
  11. Brown MS, et al. 2022. Sci Adv. 8:eabj8002. PubMed
  12. Koizumi M, et al. 2022. J Biol Chem. 298:102506. PubMed
  13. Ogishi M, et al. 2023. J Exp Med. 220: . PubMed
  14. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  15. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  16. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  17. Nettersheim FS, et al. 2023. Front Cardiovasc Med. 9:1076808. PubMed
  18. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  19. Xin DQ, et al. 2022. Neural Regen Res. 17:2238. PubMed
  20. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  21. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  22. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  23. Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed
  24. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
  25. Koh CH, et al. 2020. Cancer Immunol Res. 8:698. PubMed
  26. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  27. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  28. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  29. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  30. Wabitsch S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1166. PubMed
  31. He X, et al. 2021. Cell Rep. 36:109674. PubMed
  32. Wang D, et al. 2018. Immunity. 48:659. PubMed
  33. Zhang P et al. 2019. Cell Rep. 27(11):3117-3123 . PubMed
  34. Aldon Y, et al. 2020. J Immunol. 204:903. PubMed
  35. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  36. Almeida D, et al. 2010. J Immunol. 185:1927. PubMed
  37. del Rio ML, et al. 2021. Transl Res. Online ahead of print.. PubMed
  38. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  39. Feizi N, et al. 2021. Cell Death Dis. 12:1026. PubMed
  40. Pal D et al. 2017. eLife. 6 pii: e21615. PubMed
  41. Bhattacharjee S, et al. 2019. Cell Rep. 28:231. PubMed
  42. Gotoh K et al. 2018. Cell reports. 25(7):1800-1815 . PubMed
  43. Gondek D, et al. 2012. J Immunol. 189:2441. PubMed
  44. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  45. Li Y, et al. 2020. Nat Commun. 11:2781. PubMed
  46. Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed
  47. LaMothe RA et al. 2018. Front Immunol. 0.570138889 . PubMed
  48. Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed
  49. Bolinger B, et al. 2013. J Immunol. 190:4162. PubMed
  50. Kersh A, et al. 2014. J Immunol. 193:4429. PubMed
  51. Han Y, et al. 2019. Am J Cancer Res. 9:800. PubMed
  52. Ueda R, et al. 2009. Clin Cancer Res. 5.215972222. PubMed
  53. Ruf B, et al. 2021. Cancer Immunol Res. 9:1024. PubMed
  54. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  55. Nanou A, et al. 2020. Haematologica. . PubMed
  56. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  57. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  58. Pitsch J, et al. 2021. Ann Neurol. 89:666. PubMed
  59. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  60. Ruer-Laventie J, et al. 2020. Bio Protoc. e3531:10. PubMed
  61. Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed
  62. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  63. Ise W, et al. 2018. Immunity. 48:702. PubMed
  64. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  65. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  66. Sun L, et al. 2021. Cell Reports. 35(9):109188. PubMed
  67. Vishnoi M, et al. 2015. Sci Rep. 5: 17533. PubMed
  68. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  69. Wang D, et al. 2020. J Cell Mol Med. 24:4212. PubMed
  70. Alvim RG, et al. 2021. Molecules. 26:. PubMed
  71. Zhang Q, et al. 2022. Nat Commun. 13:2406. PubMed
  72. Anger-Góra N, et al. 2021. Oncol Lett. 22:582. PubMed
  73. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  74. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  75. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  76. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  77. Jandke A, et al. 2020. Nat Commun. 3.075694444. PubMed
  78. Chulkina M, et al. 2017. Eur J Pharmacol. 10.1016/j.ejphar.2017.06.033. PubMed
  79. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  80. Jeljeli M, et al. 2021. Front Immunol. 12:670776. PubMed
  81. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  82. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  83. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  84. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  85. Limbach K, et al. 2017. Malar J. 10.1186/s12936-017-1911-z. PubMed
  86. Yomtoubian S, et al. 2020. Cell Reports. 30(3):755-770.e6.. PubMed
  87. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  88. Salazar V, et al. 2019. Cell Rep. 26:1585. PubMed
  89. Jeon Y, et al. 2015. PLoS One. 10: e0139845. PubMed
  90. Xu W, et al. 2019. Immunity. 51(5):826-839.e5. PubMed
  91. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  92. Field CS, et al. 2020. Cell Metab. 31:422. PubMed
  93. Skirecki T, et al. 2020. JCI Insight. 5:. PubMed
  94. Wang X, et al. 2022. Elife. 11:. PubMed
  95. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  96. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  97. Kitagawa K, et al. 2014. Mol Cell Biol. 34:2732. PubMed
  98. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  99. Belogortseva N, et al. 2017. PLoS One.. 10.1371/journal.pone.0182825. PubMed
  100. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  101. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  102. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  103. Pattabiraman D, et al. 2016. Science. 351: aad3680. PubMed
  104. Miao Y et al. 2019. Cell. 177(5):1172-1186 . PubMed
  105. Pan Y, et al. 2021. NPJ Vaccines. 6:1. PubMed
  106. Aihara E, et al. 2015. Sci Rep. 5:17185. PubMed
  107. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
RRID
AB_830786 (BioLegend Cat. No. 103029)
AB_830786 (BioLegend Cat. No. 103030)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD44 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD44 IM7 FC
Biotin anti-mouse/human CD44 IM7 FC,ICC,IHC-P
FITC anti-mouse/human CD44 IM7 FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 IM7 FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44 IM7 FC
PE anti-mouse/human CD44 IM7 FC
Alexa Fluor® 488 anti-mouse/human CD44 IM7 FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44 IM7 FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44 IM7 FC
Alexa Fluor® 700 anti-mouse/human CD44 IM7 FC,SB
PE/Cyanine7 anti-mouse/human CD44 IM7 FC
APC/Cyanine7 anti-mouse/human CD44 IM7 FC
PerCP/Cyanine5.5 anti-mouse/human CD44 IM7 FC
PerCP anti-mouse/human CD44 IM7 FC
Brilliant Violet 421™ anti-mouse/human CD44 IM7 FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 785™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 510™ anti-mouse/human CD44 IM7 FC
Ultra-LEAF™ Purified anti-mouse/human CD44 IM7 FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 (Maxpar® Ready) IM7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44 IM7 ICC,FC,IHC-P,SB
PE/Dazzle™ 594 anti-mouse/human CD44 IM7 FC
Brilliant Violet 711™ anti-mouse/human CD44 IM7 FC
APC/Fire™ 750 anti-mouse/human CD44 IM7 FC
TotalSeq™-A0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-C0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-B0073 anti-mouse/human CD44 IM7 PG
Spark YG™ 570 anti-mouse/human CD44 IM7 FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44 IM7 FC
TotalSeq™-D0073 anti-mouse/human CD44 IM7 PG
Brilliant Violet 750™ anti-mouse/human CD44 IM7 FC
PerCP/Fire™ 806 anti-mouse/human CD44 IM7 FC
Spark Red™ 718 anti-mouse/human CD44 IM7 FC
PE/Fire™ 810 anti-mouse/human CD44 IM7 FC
Spark Blue™ 550 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Spark Red™ 718 anti-mouse/human CD44 (Flexi-Fluor™) IM7 FC
Go To Top Version: 2    Revision Date: 09/11/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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