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PE Donkey anti-rabbit IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4064 (See other available formats)
Regulatory Status
RUO
Isotype
Donkey Polyclonal Ig
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Product Citations
publications
Poly4064_PE_Asialo-GM1_Antibody_FC_042414.jpg
C57BL/6 splenocytes were stained with NK-1.1 APC and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) PE (top image).
  • Poly4064_PE_Asialo-GM1_Antibody_FC_042414.jpg
    C57BL/6 splenocytes were stained with NK-1.1 APC and purified rabbit anti-Asialo-GM1, followed by donkey anti-rabbit IgG (clone Poly4064) PE (top image).
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406421 100 µg $104
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Description

This polyclonal donkey anti-rabbit IgG antibody reacts with the heavy chains of rabbit IgG and with the light chains common to most rabbit immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other subclasses of rabbit immunoglobulins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rabbit
Antibody Type
Polyclonal
Host Species
Donkey
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This conjugated polyclonal donkey anti-rabbit IgG antibody is useful for immunofluorescent staining for flow cytometry, or microscopy.

Product Citations
  1. Ramovs V, et al. 2019. Breast Cancer Res. 21:63. PubMed
  2. Suzuki K, et al. 2019. Int J Mol Med. 44:1187. PubMed
  3. Eldi P, et al. 2022. Immunol Cell Biol. 100:250. PubMed
  4. Zuidema A, et al. 2022. J Cell Biol. 221: . PubMed
  5. Asashima H, et al. 2022. J Clin Invest. 132: . PubMed
  6. Zhang H, et al. 2022. Adv Sci (Weinh). :e2204463. PubMed
  7. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  8. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  9. Shukla GS, et al. 2020. Clin Transl Oncol. 22:1094. PubMed
  10. Kim EH, et al. 2020. Elife. 9:00. PubMed
  11. Komegae EN, et al. 2019. Front Immunol. 10:1496. PubMed
  12. Ramovs V, et al. 2020. J Invest Dermatol. . PubMed
  13. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  14. Hsiue EH, et al. 2021. Science. 371:. PubMed
  15. Bencze D, et al. 2022. Int J Mol Sci. 23:. PubMed
  16. Liang F, et al. 2021. Front Immunol. 12:658715. PubMed
  17. Adler D, et al. 2019. iScience. 15:39. PubMed
  18. Zuidema A, et al. 2022. J Cell Sci. 135:. PubMed
  19. Alberts A, et al. 2020. Sci Rep. 10:6391. PubMed
  20. Min H, et al. 2015. PLoS One. 10: 0138201. PubMed
  21. Ryniawec JM, et al. 2022. Diagnostics (Basel). 12:. PubMed
  22. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  23. Liu Y, et al. 2022. J Inflamm Res. 15:4315. PubMed
  24. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  25. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
  26. Kim S, et al. 2021. Elife. 10:. PubMed
  27. Routhu NK, et al. 2021. Immunity. 54(3):542-556.e9. PubMed
  28. Walsh SM, et al. 2021. eLife. 10:00. PubMed
  29. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  30. Routhu NK, et al. 2021. Nat Commun. 12:3587. PubMed
  31. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  32. Carmona-Rivera C, et al. 2020. JCI Insight. 5:00. PubMed
  33. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  34. You C, et al. 2021. Cancers (Basel). 13:. PubMed
  35. Secchia S, et al. 2022. Dev Cell. 57:496. PubMed
  36. Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed
  37. Thirugnanam K, et al. 2019. J Am Heart Assoc. 8:e012792. PubMed
  38. Zuidema A, et al. 2018. J Cell Sci. 131. PubMed
  39. Kannan S, et al. 2019. Mol Cancer Ther. 1.871527778. PubMed
  40. Fujiwara Y, et al. 2020. Clin Transl Immunology. 9:e1162. PubMed
  41. Claus M, Wingert S, Watzl C 2016. Open Bio. 6: 160010. PubMed
  42. Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed
  43. Zheng C, et al. 2021. J Extracell Vesicles. 10:e12109. PubMed
  44. Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
  45. van Nie L, et al. 2020. Sci Rep. 10:11928. PubMed
  46. Kwok HS, et al. 2019. ACS Chem Biol. 14:603. PubMed
RRID
AB_2563484 (BioLegend Cat. No. 406421)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/09/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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