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PE Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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Product Citations
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405307 200 µg $219
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The IgG antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IgG antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
  2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
  3. Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations
  1. Wu J, et al. 2022. Nat Commun. 13:676. PubMed
  2. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  3. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  4. Ban B, et al. 2022. Antibodies (Basel). 11: . PubMed
  5. Im NG, et al. 2022. Cancer Immunol Res. 10:1055. PubMed
  6. Bharadwaj P, et al. 2022. Cell Rep Med. 3:100818. PubMed
  7. Ji K, et al. 2023. Clin Transl Med. 13:e1234. PubMed
  8. Daradoumis J, et al. 2023. Viruses. 15:. PubMed
  9. Zhang G, et al. 2023. J Immunother Cancer. 11:. PubMed
  10. Ryszawy D, et al. 2014. Carcinogenesis. 35:1920. PubMed
  11. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  12. Uray K, et al. 2011. Surgery. 149:114. PubMed
  13. Griffiths K, et al. 2016. J Biol Chem. 291: 12641 - 12657. PubMed
  14. Tiper I, et al. 2016. Clin Cancer Res. 22: 4249 - 4258. PubMed
  15. Liu Y, et al. 2016. Acta Biochim Biophys Sin. 48: 909 - 922. PubMed
  16. Vanhulle E, et al. 2022. Front Cell Infect Microbiol. 12:989534. PubMed
  17. Tian M, et al. 2022. J Clin Invest. 132:. PubMed
  18. Wang Y, et al. 2020. Nat Commun. 11:860. PubMed
  19. Chittasupho C, Anuchapreeda S, and Sarisuta N. 2017. Eur J Pharm Biopharm. 10.1016/j.ejpb.2017.07.003. PubMed
  20. Byazrova MG, et al. 2022. NPJ Vaccines. 7:145. PubMed
  21. Hirata T, et al. 2022. J Biol Chem. :102444. PubMed
  22. Busnadiego I, et al. 2022. PLoS Biol. 20:e3001709. PubMed
  23. Jiang X, et al. 2021. Sci Signal. 14:. PubMed
  24. Murcia JDG, et al. 2020. Sci Rep. 10:8019. PubMed
  25. Sinha D, et al. 2021. J Immunother Cancer. 9:. PubMed
  26. Rong Y, et al. 2015. PLoS One. 10: 0138553. PubMed
  27. Hirata T, et al. 2015. Mol Biol Cell. 26: 3071-3084. PubMed
  28. Tsukamoto H, et al. 2017. FEBS Lett. 10.1002/1873-3468.12768. PubMed
  29. Pei X, et al. 2014. J Immunol. 192:1878. PubMed
  30. Petrie-Hanson L, et al. 2022. Pathogens. 11:. PubMed
  31. Parente-Pereira AC, et al. 2022. STAR Protoc. 3:101319. PubMed
  32. Becerril C, et al. 2021. Biomolecules. 11:. PubMed
  33. Kmieciak M, et al. 2011. J Vis Exp. 47: 2381. PubMed
  34. Larcombe-Young D, et al. 2022. STAR Protoc. 3:101414. PubMed
  35. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  36. Lobastova L, et al. 2021. Frontiers in Cell and Developmental Biology. 9:698503. PubMed
  37. Winter S, Bardoel A 2016. Sci Rep. 6: 24242. PubMed
  38. Kimura T, et al. 2015. J Immunol. 194:5426. PubMed
  39. Hirata T, et al. 2018. Nat Commun. 9:405. PubMed
  40. Wu YF, et al. 2019. Autophagy. :1. PubMed
  41. Gordts S, et al. 2015. PLoS One. 10: 0131219. PubMed
  42. Kissiov DU, et al. 2022. Elife. 11:. PubMed
  43. Sakaguchi K, et al. 2018. Biochem Biophys Rep. 14:89. PubMed
  44. Soday L et al. 2019. Cell reports. 27(6):1920-1933 . PubMed
  45. Gu Q, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.05.011. PubMed
  46. Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
  47. Vrijens P, et al. 2019. J Gen Virol. 100:583. PubMed
  48. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  49. Vogelgesang A, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0914-6. PubMed
  50. Tam K, et al. 2016. MBio. 7: e02024-16. PubMed
RRID
AB_315010 (BioLegend Cat. No. 405307)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All IgG Reagents Request Custom Conjugation
Description Clone Applications
APC Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Biotin Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
FITC Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
HRP Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ELISA,IHC,WB
PE Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Purified Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ELISA,FC,WB,IHC
Cyanine3 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC
PE/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PerCP/Cyanine5.5 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
DyLight™ 488 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,ICC
DyLight™ 649 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Alexa Fluor® 594 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC,IHC-F,IHC-P
APC/Cyanine7 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Brilliant Violet 421™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,ICC
Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-F
Alexa Fluor® 647 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-P
Alexa Fluor® 555 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 ICC
Brilliant Violet 605™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Brilliant Violet 510™ Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PE/Dazzle™ 594 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
PerCP Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
APC/Fire™ 750 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC
Spark YG™ 570 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 FC,IHC-P
Spark Red™ 718 Goat anti-mouse IgG (minimal x-reactivity) Poly4053 IHC-P,IHC-F,ICC
Go To Top Version: 4    Revision Date: 02/09/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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