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Purified anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody

Pricing & Availability
Clone
GL7 (See other available formats)
Regulatory Status
RUO
Other Names
Ly77, T and B cell activation marker
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
1_GL7_Purified_GL7_Antibody_FC_1_091912
Balb/c mouse bone marrow cells were stained with IgD APC and purified GL7 (clone GL7, top) or purified rat IgM, κ isotype control (bottom), followed by anti-rat IgM FITC.
  • 1_GL7_Purified_GL7_Antibody_FC_1_091912
    Balb/c mouse bone marrow cells were stained with IgD APC and purified GL7 (clone GL7, top) or purified rat IgM, κ isotype control (bottom), followed by anti-rat IgM FITC.
  • 2_GL7_Purified_GL7_Antibody_FC_2_091912
  • GL7_PURE_GL7_Antibody_3_012022
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of GL7 (clone GL7) purified, CD3 (clone 17A2) Alexa Fluor® 647 (green) and B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgM (clone MRM-47) Biotin for 2 hours, followed by 2.5 µg/ml of Streptavidin Alexa Fluor® 594 (red) for 2 hours at room temperature. The image was captured by 10X objective.
  • GL7_PURE_GL7_Antibody_4_012022
    LPS-challenged C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of GL7 (clone GL7) purified, CD3 (clone 17A2) Alexa Fluor® 647 (green) and B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C, followed by 2.5 µg/ml of anti-rat IgM (clone MRM-47) Biotin for 2 hours, followed by 2.5 µg/ml of Streptavidin Alexa Fluor® 594 (red) for 2 hours at room temperature. The image was captured by 10X objective.
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144601 50 µg $77
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144602 500 µg $293
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Description

The GL7 antigen, also known as Ly77, is a 35 kD protein.  The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes.  It is upregulated on mouse splenocytes following activation.  It may play a role in regulation or adhesion.  GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS activated DBA/J mouse B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA, IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
  2. Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
  3. Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
  4. Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
  5. Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
Product Citations
  1. Kobayashi A, et al. 2021. Front Immunol. 12:650856. PubMed
  2. Avalos A, et al. 2022. JCI Insight. 7: . PubMed
  3. Pang Z, et al. 2022. ACS Nano. 16:16019. PubMed
  4. Landuyt AE, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  5. Fonseca JA, et al. 2018. Vaccine. 36:2799. PubMed
  6. Son YM, et al. 2020. Eur J Immunol. 50:1067. PubMed
  7. Crawford G, et al. 2018. Nat Immunol. 1.388194444. PubMed
  8. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  9. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  10. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  11. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  12. Firmino NS, et al. 2021. Oncoimmunology. 10:1959978. PubMed
  13. Zelazowska MA, et al. 2020. Life Sci Alliance. :3. PubMed
  14. Papa I, et al. 2017. Nature. 547:318. PubMed
  15. Duncan CG, et al. 2018. G3 (Bethesda). 0.892361111. PubMed
  16. Prabakaran T, et al. 2021. EBioMedicine. 66:103314. PubMed
RRID
AB_2561547 (BioLegend Cat. No. 144601)
AB_2561547 (BioLegend Cat. No. 144602)

Antigen Details

Structure
35 kD
Distribution

Germinal center B cells, activated B and T cells

Function
Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
Ligand/Receptor
Neu5Ac-recognizing lectins
Cell Type
B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.

Gene ID
NA
UniProt
View information about GL7 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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