Bruker Spatial Biology presents CellScape™ Precise Spatial Multiplexing, an end-to-end immunofluorescence platform for highly multiplexed spatial proteomics and single-cell analysis. With advanced optical capabilities, streamlined fluidics for walk-away automation, and unprecedented flexibility in assay design, the CellScape system will accelerate your exploration into the rapidly evolving field of spatial biology.

CellScape technology features:

• High multiplexing capability
• Throughput and automation
• Quantitative imaging
• Workflow and reagent versatility

Multiplexed IHC staining of PerCP/Cyanine5.5 anti-CD45RO (clone UCHL1) on formalin-fixed paraffin-embedded human tonsil tissue, validated for use on the CellScape. The tissue was incubated with PerCP/Cyanine5.5 anti-CD45RO (clone UCHL1, red) and Alexa Fluor® 488 anti-CD3 (green) for one hour at room temperature. Nuclei were counterstained with Hoechst 33342. Images were captured with a 20X objective.

Multiplexed IHC staining of Alexa Fluor® 488 anti-Vimentin on formalin-fixed paraffin-embedded human tonsil tissue, validated for use on the Cellscape. The tissue was iteratively stained with Alexa Fluor® 488 anti-Vimentin (green) and Alexa Fluor® 488 anti-CD3 (red) for one hour at room temperature. Nuclei were counterstained with DAPI. Images were captured with a 20X objective.

Multiplexed IHC staining of PerCP/Cyanine5.5 anti-CD138 (clone MI15) on formalin-fixed paraffin-embedded human tonsil tissue, validated for use on the Cellscape. The tissue was incubated with PerCP/Cyanine5.5 anti-CD138 (clone MI15, green) and PerCP/Cyanine5.5 anti-CD45 (red) for one hour at room temperature. Nuclei were counterstained with Hoechst 33342. Images were captured with a 20X objective.

Multiplexed IHC staining of PerCP/Cyanine5.5 anti-CD326 on formalin-fixed paraffin-embedded human tonsil tissue, validated for use on the Cellscape. The tissue was iteratively stained with PerCP/Cyanine5.5 anti-CD326 (green) and PerCP/Cyanine5.5 anti-CD45 (red) for one hour at room temperature. Nuclei were counterstained with Hoechst 33342. Images were captured with a 20X objective.