COMET™ Scalable Hyperplexing with BioLegend Antibodies

The only high-throughput, hyperplex platform: scalability and reproducibility without the need for conjugation.

 

COMET™ is the first universal, end-to-end, spatial biology solution for the analysis of Formalin-Fixed Paraffin-Embedded (FFPE) and frozen tissues offered by Lunaphore. The COMET™ is a fully automated, high-throughput, hyperplex platform that seamlessly performs sequential immunofluorescence (seqIF™) protocols thanks to a patented precision microfluidic chip technology called Fast-Fluidic Exchange (FFeX™). COMET™ works with off-the-shelf, non-conjugated primary antibodies and fluorescent conjugated secondary antibodies, allowing users to transfer and optimize previously validated immunohistochemistry/immunofluorescence (IHC/IF) protocols with ease. Our high-quality and trusted antibodies integrate smoothly with Lunaphore’s COMET™ platform, and, through our collaboration, we aim to make your hyperplex imaging limitless.

 

Hyperplex Workflow with COMET™

 

Sequential immunofluorescence (seqIF™️) staining on COMET™ of anti-CD45RA (clone HI100, green), anti-CD34 (clone QBEND/10, magenta), anti-CD138 (clone MI15, yellow), anti-Tryptase (clone AA1, red), and anti-S100B (clone 11C12E12, white) on formalin-fixed paraffin-embedded (FFPE) human skin tissue. Alexa Fluor™ Plus 647 Goat anti-Mouse IgG antibody (Lunaphore, Cat. No. DR647MS) was used as a secondary antibody. Nuclei were counterstained with DAPI (blue). Tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing.

Sequential immunofluorescence (seqIF™️) staining on COMET™ of anti-CD45RA (clone HI100, magenta), anti-CD34 (clone QBEND/10, green), anti-CD138 (clone MI15, yellow), anti-S100B (clone 11C12E12, red), and anti-Vimentin (clone O91D3, cyan) on formalin-fixed paraffin-embedded (FFPE) human pancreatic carcinoma. Alexa Fluor™ Plus 647 Goat anti-Mouse IgG antibody (Lunaphore, Cat. No. DR647MS) was used as a secondary antibody. Nuclei were counterstained with DAPI (blue). Tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing.

Sequential immunofluorescence (seqIF™️) staining on COMET™  of anti-CD324 (clone 4A2, yellow) on formalin-fixed paraffin-embedded (FFPE) human prostate carcinoma tissue. Alexa Fluor™ Plus 647 Goat anti-Mouse IgG antibody (Lunaphore, Cat. No. DR647MS) was used as a secondary antibody. Nuclei were counterstained with DAPI (blue). Tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing.

BioLegend Antibodies and COMET

Lunaphore offers SPYRE™ Antibody Panels for human FFPE samples, targeting key markers in immuno-oncology and immunology research. These panels are designed with maximum flexibility in mind to support research needs, as they can be used as a basis for custom panel development. Using Lunaphore’s Panel Builder, an intuitive and convenient online tool, panel modules can be combined, allowing “add-on” antibodies to address specific biological questions. From our large portfolio of unconjugated primary antibodies, we are continuously working to validate antibodies for integration on the COMET™ platform. Explore our current list below to incorporate into your next seqIF™ experiment.

 

Specificity

Clone

Host

Reactivity

Working Concentration
for the COMET™ (µg/mL)

anti-mouse/human Bcl-6

IG191E/A8

Mouse

Human, Mouse

1.00

anti-CD34

QBEND/10

Mouse

Human

1.25

anti-human CD45RA

HI100

Mouse

Human

0.50

anti-human CD45RO

UCHL1

Mouse

Human

2.50

anti-human CD66b

6/40c

Mouse

Human

5.00

anti-human CD107a (LAMP-1)

H4A3

Mouse

Human

5.00

anti-human CD138 (Syndecan-1)

MI15

Mouse

Human

5.00

anti-human CD163 Recombinant

QA19A16

Mouse

Human

5.00

anti-human CD235ab

HIR2

Mouse

Human

0.83

anti-human/mouse/rat CD278 (ICOS)

C398.4A

ArHam

Human, Mouse, Rat

5.00

anti-CD324 (E-Cadherin)

4A2

Mouse

Human, Rat

5.00

anti-human FOXP3

259D

Mouse

Human

10.00

anti-Granzyme B

M3304B06

Mouse

Human

5.00

anti-human HLA-DR

LN3

Mouse

Human

2.50

anti-IDO1

W16073A

Rat

Human

10.00

anti-human IgD

W18340A

Rat

Human

5.00

anti-human Mast Cell Tryptase

AA1

Mouse

Human

0.06

anti-human Podoplanin

NC-08

Rat

Human

5.00

anti-S100B

11C12E12

Mouse

Human, Mouse, Rat

1.25

anti-Vimentin

O91D3

Mouse

Human

0.33

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