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Purified anti-human Podoplanin Antibody

Pricing & Availability
Clone
NC-08 (See other available formats)
Regulatory Status
RUO
Other Names
T1a, T1a2, gp36, gp38, gp40, Aggrus, PDPN, D2-40
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
A_NC-08_PURE_Podoplanin_2_12142022
IHC staining of Purified anti-human Podoplanin (clone NC-08) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Tris-EDTA (10mM Tris, 1mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-rat IgG antibody (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 20X objective. Scale bar: 50 µm
  • A_NC-08_PURE_Podoplanin_2_12142022
    IHC staining of Purified anti-human Podoplanin (clone NC-08) on formalin-fixed paraffin-embedded human placenta tissue. Following antigen retrieval using Tris-EDTA (10mM Tris, 1mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-rat IgG antibody (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 20X objective. Scale bar: 50 µm
  • B_NC-08_PURE_Podoplanin_3_12142022
    IHC staining of Purified anti-human Podoplanin (clone NC-08) on formalin-fixed paraffin-embedded human breast tissue. Following antigen retrieval using 1X Tris-Buffered Saline (final concentration 0.05M) with Tris-EDTA (10mM Tris, 1mM EDTA, 0.05% Tween-20, pH 9.0), the tissue was incubated without (panel A) and with (panel B) 10 µg/mL of primary antibody followed by incubation with Alexa Fluor® 647 goat anti-rat IgG antibody (Cat. No. 405416) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 20X objective. Scale bar: 50 µm
  • C_NC08_PURE_Podoplanin_Antibody_SB_111423
    SeqIF™ (sequential immunofluorescence) staining on COMET™ of Purified anti-Podoplanin (clone NC-08, yellow) on formalin-fixed paraffin-embedded human skin tissue at 5 µg/mL. Alexa Fluor™ Plus 555 Goat anti-Rat IgG antibody (Lunaphore, Cat. No. DR555RT) was used as a secondary antibody. Nuclei were counterstained with DAPI (blue). Tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing.
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337001 25 µg $112
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337002 100 µg $229
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Description

Podoplanin is a 40-43 kD type-I transmembrane sialomucin-type glycoprotein, also known as T1a, gp36, gp38, gp40, and Aggrus. Originally detected on the surface of podocytes, futher characterization showed podoplanin has a broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and a variety of tumor cells. It has been reported that podoplanin is the ligand of CLEC2 and is involved in lymphatic vessel formation, platelet aggregation, and tumor metastasis. Podoplanin may serve as a useful marker for tumor diagnosis and prognosis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-P - Verified
ICC - Reported in the literature, not verified in house 
SB - Community verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence1.

Additional Product Notes

For the use of this antibody in spatial biology applications, we have partnered with Lunaphore Technologies for demonstration of our antibodies on the COMET™. The COMET™ platform is an automated, end-to-end spatial biology solution developed for rapid and flexible multiplex tissue profiling. More information on the COMET™ and a complete list of our antibodies that have been demonstrated on the COMET™ can be found here.

Application References

(PubMed link indicates BioLegend citation)
  1. Fujino N, et al. 2012. Am. J. Respir. Cell. Mol. Biol. 46:422. (FC, IF)
Product Citations
  1. Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed
  2. Mizuta K, et al. 2022. EMBO J. 41:e110815. PubMed
  3. Chyou S, et al. 2011. J Immunol. 187:5558. PubMed
  4. Kamiya Y, et al. 2021. PLoS One. 16:e0249367. PubMed
  5. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  6. Labib D, et al. 2022. Front Mol Neurosci. 15:870085. PubMed
RRID
AB_1595616 (BioLegend Cat. No. 337001)
AB_1595616 (BioLegend Cat. No. 337002)

Antigen Details

Structure
40-43 kD type-I transmembrane sialomucin-type glycoprotein
Distribution

Broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and many tumor cells

Function
Lymphatic vessel formation, tumor metastasis, platelet aggregation
Ligand/Receptor
CLEC2
Cell Type
Dendritic cells
Biology Area
Cancer Biomarkers, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References

1. Raica M, et al. 2008. Anticancer Res. 28:2997.
2. Xie Q, et al. 2008. Int. J. Clin. Exp. Pathol. 1:276.
3. Ogasawara S, et al. 2008. Hybridoma. 27:259.
4. Kato Y, et al. 2003. J. Bio. Chem. 278:51599.

Gene ID
10630 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Go To Top Version: 6    Revision Date: 11/14/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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